Prevention of fluid delivered to reservoir from wicking into channels within microfluidic device

• US 8,252,160 B2
• Filed: 07/28/2006
• Issued: 08/28/2012
• Est. Priority Date: 07/28/2006
• Status: Expired due to Fees

• Current Assignee: Hewlett-Packard Development Company, L.P. (HP Inc.)
• Original Assignee: Hewlett-Packard Development Company, L.P. (HP Inc.)
• Inventors: Harding, Philip
• Primary Examiner(s): Ramdhanie, Bobby
• Application Number:
  US11/460,627
• Publication Number:
  US 20080025873A1
• Time in Patent Office: 2,223 Days
• Field of Search: 422/100, 422/500
• US Class Current: 204/500
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2300/069 Absorbents; Gels to retain a fluid
• CPC Class Codes: B01L 2300/0803 Disc shape
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

A microfluidic device includes a substrate and a non-valve capillary mechanism. At least a reservoir and one or more channels leading to the reservoir are formed within the substrate. The non-valve capillary mechanism is within the reservoir, and prevents fluid delivered to the reservoir from wicking from the reservoir into the channels.

Claims

1. A microfluidic device comprising:

a substrate having formed therein at least a reservoir to which one or more channels lead; and

,

a non-valve backflow-prevention mechanism within the reservoir to prevent fluid delivered to the reservoir via the channels from flowing back from the reservoir into the channels, the non-valve backflow-prevention mechanism comprising a capillary medium.

2. The microfluidic device of claim 1, wherein the capillary medium comprises a non-swelling capillary medium apart from the substrate and inserted into the reservoir.

3. The microfluidic device of claim 2, wherein the non-swelling capillary medium comprises a plurality of interstices smaller in relation to the channels, the fluid retained within the interstices of the medium inserted into the reservoir such that the fluid is prevented from wicking from the reservoir into the channels, the fluid otherwise unabsorbed within the medium itself.

4. The microfluidic device of claim 1, wherein the capillary medium comprises a swelling capillary medium apart from the substrate and inserted into the reservoir.

5. The microfluidic device of claim 4, wherein the swelling capillary medium comprises a plurality of interstices smaller in relation to the channels, the fluid retained within the interstices of the medium inserted into the reservoir and absorbed within the medium itself such that the fluid is prevented from wicking from the reservoir into the channels.

6. The microfluidic device of claim 1, wherein the capillary medium comprises one or more capillaries fabricated within the substrate itself, the capillaries smaller in relation to the channels, the fluid retained within the capillaries of the mechanism such that the fluid is prevented from wicking from the reservoir into the channels.

7. The microfluidic device of claim 1, wherein the substrate is of a hydrophobic material at least at the channels and the reservoir thereof, and that becomes at least partially hydrophilic at the channels thereof upon the fluid being delivered via the channels to the reservoir.

8. The microfluidic device of claim 7, wherein the hydrophobic material of the substrate at the channels is identical to the hydrophobic material of the substrate at the reservoir.

9. The microfluidic device of claim 1, wherein the substrate is rotatable, such that rotation of the substrate causes the fluid to be delivered to the reservoir through the channels via centrifugal force, and the non-valve capillary mechanism is adapted to prevent the fluid from wicking from the reservoir back into the channels upon cessation of rotation of the substrate.

10. The microfluidic device of claim 1, wherein the microfluidic device is a microfluidic coupon device in which at least a sample and a reagent is deposited for analysis of the sample at least via assaying.

11. The microfluidic device of claim 1, wherein the channels are inlet channels via which the fluid is delivered to the reservoir, and the substrate further has formed therein one or more outlet channels via which the fluid is delivered from the reservoir.

12. A microfluidic coupon device comprising:

a substrate;

one or more reaction chambers formed within substrate and at which samples are analyzed after delivery thereto;

one or more waste reservoirs formed within the substrate, corresponding to the reaction chambers, and at least to which the samples are delivered after analysis within the reaction chambers and/or to which excess of the samples are delivered;

a network of channels to deliver the samples and/or reagents to the reaction chambers and to the waste reservoirs; and

,

one or more non-valve backflow-prevention mechanisms within and corresponding to the waste reservoirs to prevent the samples and/or the reagents delivered to the waste reservoirs via the channels from flowing from the waste reservoirs back into the channels, the non-valve backflow-prevention mechanisms comprising capillary media.

13. The microfluidic coupon device of claim 12, wherein at least one of the capillary media comprises a non-swelling capillary medium apart from the substrate and inserted into the waste reservoir corresponding to the non-valve capillary mechanism.

14. The microfluidic coupon device of claim 13, wherein the non-swelling capillary medium comprises a plurality of interstices smaller in relation to the channels, the samples and/or the reagents retained within the interstices of the medium inserted into the waste reservoir such that the samples and/or the reagents are prevented from wicking from the waste reservoir back into the channels, the samples and/or the reagents otherwise unabsorbed within the medium itself.

15. The microfluidic coupon device of claim 12, wherein at least one of the capillary media comprises a swelling capillary medium apart from the substrate and inserted into the waste reservoir corresponding to the non-valve capillary mechanism.

16. The microfluidic coupon device of claim 15, wherein the swelling capillary medium comprises a plurality of interstices smaller in relation to the channels, the samples and/or the reagents retained within the interstices of the medium inserted into the waste reservoir and absorbed within the medium itself such that the samples and/or the reagents are prevented from wicking from the waste reservoir back into the channels.

17. The microfluidic coupon device of claim 12, wherein at least one of the capillary media comprises one or more capillaries fabricated within the substrate itself, the capillaries smaller in relation to the channels, the samples and/or the reagents retained within the capillaries of the mechanism such that the samples and/or the reagents are prevented from wicking from the waste reservoir back into the channels.

18. The microfluidic coupon device of claim 12, wherein the substrate is of a hydrophobic material at least at the channels and the waste reservoirs thereof, and that becomes at least partially hydrophilic at the channels thereof upon the samples and/or the reagents being delivered via the channels to the waste reservoirs.

19. The microfluidic coupon device of claim 12, wherein the substrate is rotatable, such that rotation of the substrate causes the samples and/or reagents to be delivered to the reaction chambers and to the waste reservoirs via the channels via centrifugal force, and the non-valve capillary mechanisms are adapted to prevent the samples and/or the reagents from wicking from the waste reservoirs back into the channels upon cessation of rotation of the substrate.

20. A method comprising:

depositing a fluid onto a microfluidic device;

delivering the fluid via one or more channels formed within the microfluidic device ultimately to a reservoir formed within the microfluidic device; and

,

preventing the fluid from flowing from the reservoir back into the channels, using a non-valve backflow-prevention mechanism comprising a capillary medium,

wherein the capillary medium comprises one or more of;

a non-swelling capillary medium apart from the substrate and inserted into the reservoir;

a swelling capillary medium apart from the substrate and inserted into the reservoir; and

,

one or more capillaries fabricated within the substrate itself, the capillaries smaller in relation to the channels, the fluid retained within the capillaries of the mechanism such that the fluid is prevented from wicking from the reservoir back into the channels.