Method for inducing differentiation into cardiomyocytes using G-CSF
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1. A method for inducing differentiation of ES cells into cardiomyocytes in vitro, which comprises culturing the ES cells in the presence of an agonist for G-CSF receptor, wherein the agonist for G-CSF receptor is added to the cell culture solution 5 to 8 days after removal of leukemia inhibitory factor (LIF) from the culture medium.
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Abstract
A method for inducing differentiation of ES cells into cardiomyocytes, which comprises contacting the ES cells with an agonist for G-CSF receptor.
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11 Claims
- 1. A method for inducing differentiation of ES cells into cardiomyocytes in vitro, which comprises culturing the ES cells in the presence of an agonist for G-CSF receptor, wherein the agonist for G-CSF receptor is added to the cell culture solution 5 to 8 days after removal of leukemia inhibitory factor (LIF) from the culture medium.
- 6. A method for producing cardiomyocytes in vitro, which comprises culturing ES cells in the presence of an agonist for G-CSF receptor, wherein the agonist for G-CSF receptor is added to the cell culture solution 5 to 8 days after removal of leukemia inhibitory factor (LIF) from the culture medium.
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