Modular nucleotide compositions and uses therefor

• US 8,252,910 B2
• Filed: 11/18/2009
• Issued: 08/28/2012
• Est. Priority Date: 11/19/2008
• Status: Active Grant

• Current Assignee: Pacific Bioscience of California Incorporated (L'Oreal SA)
• Original Assignee: Pacific Bioscience of California Incorporated (L'Oreal SA)
• Inventors: Korlach, Jonas, Wegener, Jeffrey
• Primary Examiner(s): Riley, Jezia
• Application Number:
  US12/621,352
• Publication Number:
  US 20100152424A1
• Time in Patent Office: 1,014 Days
• Field of Search: 536/4.1, 536/25.3, 422/68.1
• US Class Current: 536/4.1
• CPC Class Codes: C07H 19/10 with the saccharide radical esterified by phosphoric or polyphosphoric acids
• CPC Class Codes: C12Q 1/6818 involving interaction of two or more labels, e.g. resonant energy transfer
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: C12Q 1/6874 involving nucleic acid arrays, e.g. sequencing by hybridisation
• CPC Class Codes: G01N 33/5308 for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites

Abstract

Nucleic acid compositions, methods of making and using such compositions that comprise modular functional groups that can be configured to provide desired functionality to different nucleotide types through a swappable and preferably non-covalent linkage component. Such compositions are useful in a variety of applications including nucleic acid analyses.

Claims

1. A composition, comprising:

a nucleoside polyphosphate and a functional group coupled to the nucleoside polyphosphate through a phosphate group;

wherein the functional group is coupled to the phosphate group by a non-covalent linkage.

2. The composition of claim 1, wherein the functional group is coupled to the nucleoside polyphosphate through a phosphate group other than an alpha phosphate group.

3. The composition of claim 1, wherein the functional group comprises a detectable label.

4. The composition of claim 3, wherein the detectable label is a fluorescent label.

5. The composition of claim 1, wherein the functional group is a payload delivered by the composition.

6. The composition of claim 1, wherein the non-covalent linkage comprises an affinity linkage.

7. The composition of claim 1, wherein the non-covalent linkage comprises a biotin group.

8. The composition of claim 1, wherein the non-covalent linkage comprises one of avidin, streptavidin, neutravidin or a biotin-binding subunit thereof.

9. The composition of claim 1, wherein the non-covalent linkage comprises an antibody or a fraction thereof.

10. The composition of claim 1, wherein the non-covalent linkage comprises a polynucleotide.

11. The composition of claim 1, wherein the non-covalent linkage comprises a nucleic acid binding protein.

12. The composition of claim 1, wherein the non-covalent linkage is a polyvalent non-covalent linkage.

13. The composition of claim 12, comprising at least first and second functional groups coupled to the nucleoside polyphosphate through the polyvalent non-covalent linkage.

14. The composition of claim 13, wherein the at least first and second functional groups are the same.

15. The composition of claim 13, wherein the at least first and second functional groups are different.

16. The composition of claim 15, wherein the at least first and second functional groups comprise spectrally distinguishable fluorescent labels.

17. The composition of claim 15, wherein the first and second functional groups comprise different charges.

18. The composition of claim 12, comprising at least first and second nucleoside polyphosphates coupled to the functional group through the polyvalent non-covalent linkage.

19. The composition of claim 1, comprising at least a first and second non-covalent linkage that couple at least a first and a second nucleoside polyphosphate to the functional group.

20. The composition of claim 1, comprising at least a first and second non-covalent linkage that couple the nucleoside polyphosphate to at least a first and a second functional group.

21. The composition of claim 1, comprising up to four functional groups coupled to the nucleoside polyphosphate through a non-covalent linkage.

22. The composition of claim 1, wherein the functional group comprises a particle.

23. The composition of claim 22, wherein the particle is selected from a magnetic particle, a fluorescent semiconductor particle, a metal particle, and a polymeric particle.

24. A composition comprising the structure:

B-S-P-L-F

wherein B refers to a nucleobase moiety;

S comprises a sugar moiety, an acyclic moiety or a carbocyclic moiety;

P comprises a polyphosphate group;

L comprises a non-covalent linkage component; and

F comprises a desired functional group.

25. The composition of claim 24, wherein L comprises an affinity binding pair.