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Integrated active flux microfluidic devices and methods

  • US 8,257,666 B2
  • Filed: 02/08/2012
  • Issued: 09/04/2012
  • Est. Priority Date: 06/05/2000
  • Status: Expired due to Term
First Claim
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1. A method for analyzing a cell, the method comprising:

  • (a) introducing a fluid comprising the cell onto a microfluidic device;

    (b) lysing the cell on the microfluidic device to release cell material from the cell; and

    (c) circulating at least some of the cell material in a loop path on the microfluidic device, such that said cell material circulates multiple times though the loop path, the microfluidic device further comprising a peristaltic pump to circulate cell material through the loop path, wherein said peristaltic pump comprises at least three microvalves actuated in a coordinated fashion to move the cell material through the loop path.

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