Integrated active flux microfluidic devices and methods
First Claim
1. A method for analyzing a cell, the method comprising:
- (a) introducing a fluid comprising the cell onto a microfluidic device;
(b) lysing the cell on the microfluidic device to release cell material from the cell; and
(c) circulating at least some of the cell material in a loop path on the microfluidic device, such that said cell material circulates multiple times though the loop path, the microfluidic device further comprising a peristaltic pump to circulate cell material through the loop path, wherein said peristaltic pump comprises at least three microvalves actuated in a coordinated fashion to move the cell material through the loop path.
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Abstract
The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e. Polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene.
199 Citations
9 Claims
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1. A method for analyzing a cell, the method comprising:
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(a) introducing a fluid comprising the cell onto a microfluidic device; (b) lysing the cell on the microfluidic device to release cell material from the cell; and (c) circulating at least some of the cell material in a loop path on the microfluidic device, such that said cell material circulates multiple times though the loop path, the microfluidic device further comprising a peristaltic pump to circulate cell material through the loop path, wherein said peristaltic pump comprises at least three microvalves actuated in a coordinated fashion to move the cell material through the loop path. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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Specification