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Bisulfite conversion of DNA

  • US 8,257,950 B2
  • Filed: 06/13/2011
  • Issued: 09/04/2012
  • Est. Priority Date: 06/19/2000
  • Status: Expired due to Fees
First Claim
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1. A method for bisulfite conversion of DNA, comprising reacting isolated genomic DNA with a bisulfite reagent, wherein the reaction is conducted at a temperature in the range of 0-80°

  • C., and wherein the reaction temperature is increased at least once to a range of about 85-100°

    C. for a duration of between about 15 seconds and 6 minutes during the course of the conversion.

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