Calibration surface method for determination on of analyte ratios
First Claim
1. A method for determining a ratio of concentrations of different analytes in a sample, said method comprising:
- measuring responses of two or more analytes in a sample using a selected measuring process to obtain two or more individual analyte responses of the two or more analytes;
selecting one or more conversion methods that define a ratio of the concentrations of the two or more analytes as a direct function of the two or more individual analyte responses obtained using the selected measuring process, wherein the direct function includes a multi-variable regression model that outputs the ratio of the concentrations, and wherein the multiple variables of the regression model include the two or more individual analyte responses; and
computing, with a processor, the ratio of the concentrations of the two or more analytes in the sample using the selected one or more conversion methods and using the two or more individual analyte responses.
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Abstract
The ratio of analytes is determined directly from the responses of the analytes using a conversion method. Individual analyte responses are obtained by using a selected measuring technique, and these individual responses are used as the independent variables in a conversion method. The dependent variable of conversion method is the desired analyte ratio. The resulting conversion method is then used to directly calculate the desired ratio of analytes as a function of the measured responses. No intermediate conversions, such as using a calibration curve to convert individual measured analyte responses to concentration values, are needed to obtain the desired ratio.
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Citations
22 Claims
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1. A method for determining a ratio of concentrations of different analytes in a sample, said method comprising:
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measuring responses of two or more analytes in a sample using a selected measuring process to obtain two or more individual analyte responses of the two or more analytes; selecting one or more conversion methods that define a ratio of the concentrations of the two or more analytes as a direct function of the two or more individual analyte responses obtained using the selected measuring process, wherein the direct function includes a multi-variable regression model that outputs the ratio of the concentrations, and wherein the multiple variables of the regression model include the two or more individual analyte responses; and computing, with a processor, the ratio of the concentrations of the two or more analytes in the sample using the selected one or more conversion methods and using the two or more individual analyte responses. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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20. An apparatus for measuring a ratio of concentrations of two or more analytes, said apparatus comprising:
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a measuring module capable of measuring responses of the two or more analytes in a sample; a memory to store the measured analyte responses from the measuring module; a computer readable medium containing computer readable code having instructions for executing one or more conversion methods that defines the ratio of the concentrations of the two or more analytes as a direct function of the measured responses of the analytes obtained from the measuring module, wherein the direct function includes a multi-variable regression model that outputs the ratio of the concentrations, and wherein the multiple variables of the regression model include the two or more analyte responses; and a processor to execute the computer readable code on the computer readable medium in order to calculate the ratio of the concentrations of two or more analytes using the one or more conversion methods. - View Dependent Claims (21)
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22. A non-transitory computer-readable medium containing computer readable code having instructions for determining a ratio of concentrations of different analytes in a sample, the code comprising:
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code for measuring responses of two or more analytes in a sample using a selected measuring process to obtain two or more individual analyte responses of the two or more analytes; code for selecting a conversion method that defines the ratio of the concentrations of the two or more analytes as a direct function of the two or more individual responses of the analytes obtained from the selected measuring process, wherein the direct function includes a multi-variable regression model that outputs the ratio of the concentrations, and wherein the multiple variables of the regression model include the two or more analyte responses; and code for computing the ratio of the concentrations of the two or more the analytes of the sample using the selected conversion method and using the measured analyte responses from the sample.
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Specification