Software for the display of chromatographic separation data

• US 8,260,561 B2
• Filed: 02/24/2011
• Issued: 09/04/2012
• Est. Priority Date: 12/30/1997
• Status: Expired due to Term

• Current Assignee: Caliper Life Sciences Incorporated (Revvity, Inc.)
• Original Assignee: Caliper Life Sciences Incorporated (Revvity, Inc.)
• Inventors: Gallagher, Steven J.
• Primary Examiner(s): Lau, Tung S
• Application Number:
  US13/034,465
• Publication Number:
  US 20110144923A1
• Time in Patent Office: 558 Days
• Field of Search: 702/32
• US Class Current: 702/32
• CPC Class Codes: G01N 2030/625 by measuring reference material, e.g. carrier without sample
• CPC Class Codes: G01N 27/44717 Arrangements for investigating the separated zones, e.g. localising zones
• CPC Class Codes: G01N 30/86 Signal analysis
• CPC Class Codes: G01N 30/8651 Recording, data aquisition, archiving and storage

Abstract

Techniques and systems for displaying chromatographic data using a graphical user interface are provided. Chromatographic separation data that represent a series of measurements for multiple samples detected at a single point along the length of a channel as they move through the channel past that point can be displayed on a display device of a computer system as a series of bands, the bands being arranged to resemble output from an electrophoresis gel. The bands may be aligned using a marker included in each sample.

Claims

1. A computer implemented method of displaying chromatographic separation data, comprising:

acquiring, using a separation system comprising a detector and a computer system, a first series of measurements for a first sample, wherein the first series of measurements indicates the presence of constituents in the first sample, the first sample constituents detected at a single point along the length of a channel as they move through the channel past that point;

displaying, using the computer system, the first series of measurements for the first sample as a first lane comprising a first series of bands;

acquiring, using the separation system, a second series of measurements for a second sample, the second sample constituents detected at the single point along the length of the channel as they move through the channel past that point; and

displaying, using the computer system, the second series of measurements for the second sample as a second lane comprising a second series of bands, wherein the first lane and the second lane are displayed as a parallel set of lanes.

2. The computer-implemented method of claim 1, further comprising:

receiving input to invert the display of the first and second series of bands; and

inverting the displayed first and second series of bands.

3. The computer-implemented method of claim 1, wherein the first and second samples include a marker and wherein each of the first and second series of measurements indicates presence of the marker, the marker detected at the single point along the length of the channel as it moves through the channel past that point.

4. The computer-implemented method of claim 3, the method further comprising:

identifying one or more bands in the first series of bands that correspond to the marker;

identifying one or more bands in the second series of bands that correspond to the marker;

aligning the one or more bands in the first series of bands that correspond to the marker and the one or more bands in the second series of bands that correspond to the marker with a predetermined location, thereby aligning the first and second series of bands to a common baseline.

5. The computer-implemented method of claim 3, the method further comprising:

displaying the one or more bands that correspond to the marker in each sample visually different from the bands that correspond to the constituents in each sample.

6. The computer-implemented method of claim 1, wherein the first series of measurements and the second series of measurements comprise separation data obtained from a process carried out on a microfluidic device.

7. A system comprising:

a device on which at least first and second samples are subjected to a chromatographic separation process;

an instrument configured to receive the device, the instrument comprising a detection system that detects results of the chromatographic separation process and instrumentation for controlling fluid or material transport and direction within the device; and

a computer system that displays chromatographic separation data corresponding to results received from the instrument, wherein the computer system comprises;

a processor; and

a non-transitory computer readable medium coupled to the processor that stores a computer program that presents the chromatographic separation data from each sample as a lane comprising a series of bands;

wherein the computer program presents a first lane comprising a series of bands from chromatographic separation data from the first sample and a second lane comprising a series of bands from chromatographic separation data from the second sample as a parallel set of lanes;

and wherein the computer program also identifies a band in the first series of bands that corresponds to a marker contained in the first sample, identifies a band in the second series of bands that corresponds to the same marker contained in the second sample, and aligns the bands when presenting the first and second lanes as a parallel set of lanes.