Augmentation and repair of vocal cord tissue defects

• US 8,261,749 B2
• Filed: 08/19/2008
• Issued: 09/11/2012
• Est. Priority Date: 02/20/1997
• Status: Expired due to Fees

• Current Assignee: Castle Creek Biosciences LLC (Castle Creek Biosciences, Inc.)
• Original Assignee: Fibrocell Science, Inc.
• Inventors: Keller, Gregory S.
• Primary Examiner(s): Matthews, William H.
• Application Number:
  US12/194,316
• Publication Number:
  US 20080311089A1
• Time in Patent Office: 1,484 Days
• Field of Search: 623/14.11, 623/23.64, 623 2372- 2376, 128/898, 424/93.7, 424/93.3
• US Class Current: 128/898
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61L 27/24 Collagen
• CPC Class Codes: A61L 27/3633 Extracellular matrix [ECM]
• CPC Class Codes: A61L 27/3641 characterised by the site of application in the body materials for artificial blood vessels A61L27/507; materials for use in artificial skin A61L27/60
• CPC Class Codes: A61L 27/3683 subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
• CPC Class Codes: A61L 27/3804 characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
• CPC Class Codes: A61L 27/3839 characterised by the site of application in the body materials for artificial blood vessels A61L27/507; materials for use in artificial skin A61L27/60
• CPC Class Codes: A61L 27/3895 using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
• CPC Class Codes: A61P 11/04 for throat disorders
• CPC Class Codes: C12N 2533/54 Collagen; Gelatin
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: C12N 5/0653 Adipocytes; Adipose tissue
• CPC Class Codes: C12N 5/0656 Adult fibroblasts

Abstract

A method for corrective surgery in a human subject of a vocal cord defect amenable to rectification by the augmentation of tissue subadjacent to the vocal cord defect, the method comprising: injecting an effective amount of autologous in vitro cultured fibroblast cells into the lamina propia, Reinke'"'"'s space, the lateral tissue or the lateral edge of the vocal cord tissue of the subject in a position subadjacent to the vocal cord defect, wherein the in vitro fibroblast cultured cells are retrieved from the subject.

Claims

1. A method for corrective surgery in a human subject of a vocal cord defect amenable to rectification by the augmentation of tissue subadjacent to the vocal cord defect, the method comprising:

injecting an effective amount of autologous in vitro cultured fibroblast cells into the lamina propria, Reinke'"'"'s space, the lateral tissue or the lateral edge of the vocal cord tissue of the subject in a position subadjacent to the vocal cord defect,

wherein the in vitro fibroblast cultured cells are obtained by culturing a plurality of viable cells retrieved from the subject.

2. The method of claim 1 wherein the fibroblasts are derived from a tissue selected from the group consisting of dermis, fascia, connective tissue, and lamina propria.

3. The method of claim 1 wherein serum from the subject is used for the in vitro culture of cells.

4. The method of claim 1 wherein the in vitro cultured cells are washed in phosphate buffered saline before being placed into the tissue of the subject.