Method for stabilizing leuco-type colorant
First Claim
1. A method of suppressing nonspecific color development in a reaction liquid, the method comprisingcombining a dye mixture comprisinga leuco dye and a different dye having an absorption spectrum not influencing a measurement wavelength of the leuco dye and not reacting with hydrogen peroxide,with hydrogen peroxide and peroxidase, wherein the molar ratio of the different dye to the leuco dye is at least 0.8:
- 1 and no greater than 41.7;
1, and wherein the mixture of the different dye with the leuco dye suppresses nonspecific color development in the reaction liquid;
wherein the leuco dye comprises 10-(carboxymethylaminocarbonyl)-3,7-bis(dimethylamino)phenothiazine sodium or N,N,N′
,N′
,N″
,N″
-hexa(3-sulfopropyl)-4,4′
,4″
-triaminotriphenylmethane.6 sodium; and
wherein the different dye is selected from the group consisting of Orange G, Food Red No. 2, Food Red No. 40, Food Red No. 102 and Food Yellow No. 4.
1 Assignment
0 Petitions
Accused Products
Abstract
Provided are a method of stabilizing a leuco dye storable as a liquid for a long time, a method of reducing nonspecific color development thereof at a time of a color development reaction, and a stable liquid reagent composition using the methods. The inventors of the present invention found that coexistence of the leuco dye with a specific reducing agent resulted in suppression of self color development and its remarkably improved stability in a solution, and that when a color development reaction of the leuco dye with hydrogen peroxide was performed, coexistence of the leuco dye, in a reaction solution, with another dye which had an absorption spectrum not influencing a measurement wavelength of the leuco dye and did not react with hydrogen peroxide suppressed nonspecific color development and lowered a reagent blank value, which was applied to an analytical reagent.
35 Citations
12 Claims
-
1. A method of suppressing nonspecific color development in a reaction liquid, the method comprising
combining a dye mixture comprising a leuco dye and a different dye having an absorption spectrum not influencing a measurement wavelength of the leuco dye and not reacting with hydrogen peroxide, with hydrogen peroxide and peroxidase, wherein the molar ratio of the different dye to the leuco dye is at least 0.8: - 1 and no greater than 41.7;
1, and wherein the mixture of the different dye with the leuco dye suppresses nonspecific color development in the reaction liquid;
wherein the leuco dye comprises 10-(carboxymethylaminocarbonyl)-3,7-bis(dimethylamino)phenothiazine sodium or N,N,N′
,N′
,N″
,N″
-hexa(3-sulfopropyl)-4,4′
,4″
-triaminotriphenylmethane.6 sodium; andwherein the different dye is selected from the group consisting of Orange G, Food Red No. 2, Food Red No. 40, Food Red No. 102 and Food Yellow No. 4. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
- 1 and no greater than 41.7;
Specification