Pipette core member, pipette, and pipette device

• US 8,268,261 B2
• Filed: 11/22/2007
• Issued: 09/18/2012
• Est. Priority Date: 11/22/2006
• Status: Expired due to Fees

• Current Assignee: Kazusa DNA Research Institute, Nepa Gene Co., Ltd.
• Original Assignee: Altair Corporation, Kazusa DNA Research Institute, Nano Fusion Technologies, Inc.
• Inventors: Hirafuji, Mamoru, Tominaga, Ichiro, Eda, Shigeto, Yanagisawa, Ichiro, Ohara, Osamu
• Primary Examiner(s): Gordon, Brian R
• Application Number:
  US12/515,846
• Publication Number:
  US 20090317304A1
• Time in Patent Office: 1,762 Days
• Field of Search: 422/63, 422/119, 422/292, 422/515, 422/520, 422/521, 422501-505, 422/512, 422/516, 422/518, 422/509, 422/522, 738/633.2, 738/640.1, 738/641.3, 738/642.3, 738/642.4, D24/112, D24/113, D24/222, 137/803, 137/825, 204/600
• US Class Current: 422/501
• CPC Class Codes: B01L 3/022 Capillary pipettes, i.e. having very small bore B01L3/0224 - B01L3/0237 take precedence
• CPC Class Codes: B01L 3/0227 Details of motor drive means B01L3/0231, B01L3/0234 take precedence
• CPC Class Codes: C12M 33/04 by injection or suction, e.g. using pipettes, syringes, needles pipettes in general B01L3/02

Abstract

A pipette core member is used in a pipette for sampling a sample. This pipette core member includes: a pump body including a reservoir communicated with one suction/discharge port of an electroosmotic flow pump, the electroosmotic flow pump and the reservoir being integrally formed; and a capillary connected to the electroosmotic flow pump and communicated with the other suction/discharge port of the electroosmotic flow pump. The capillary is secured to the pump body.

Claims

1. A pipette device comprising:

a pipette comprising;

a pipette body; and

a pipette core member retained inside the pipette body, the pipette core member comprising;

a pump body comprising;

an electroosmotic flow pump having a first suction/discharge port and a second suction/discharge port, wherein the electroosmotic flow pump further comprises a first electrode, a second electrode, and an electroosmotic material sandwiched between the first electrode and the second electrode; and

a reservoir integrally formed with the electroosmotic flow pump, and configured to store a driving liquid and in liquid communication with the first suction/discharge port of the electroosmotic flow pump; and

a capillary secured to the pump body and connected to the electroosmotic flow pump, wherein the capillary also communicates with the second suction/discharge port of the electroosmotic flow pump;

a controller having a setting device that sets a voltage value of a control signal output that is used for retaining a sample in a tip end of the capillary of the pipette, wherein the setting device comprises;

a switch device that switches polarity of the voltage value of the control signal output applied to the electroosmotic flow pump; and

a multiplying factor setting device configured to multiply the voltage value by a predetermined multiplying factor; and

a control signal line that electronically connects the electroosmotic flow pump and the controller and transmits the control signal output from the controller to the electroosmotic flow pump.

2. The pipette device according to claim 1, wherein the capillary is connected to the electroosmotic flow pump via a tube connecting the electroosmotic flow pump and the capillary.

3. The pipette device according to claim 1, wherein a flow path volume from the second suction/discharge port to a tip end of the capillary is 50 microliters or below.

4. The pipette device according to claim 1, wherein a length from the second suction/discharge port to the tip end of the capillary is 18 centimeters or below.

5. The pipette device according to claim 1, wherein the pump body is formed with a length of 20 millimeters or below in an alignment direction of the electroosmotic flow pump and the reservoir, and a diameter of 8 millimeters or below around a central axis along the alignment direction.

6. The pipette device according to claim 1, wherein the pump body is formed with a weight of 1 gram or below.

7. The pipette device according to claim 1, wherein the pipette body has a cylindrical housing with a tapered end, and holds the pipette core member therein with the tip end of the capillary extending outward from the tapered end of the pipette body in an axial direction of the cylindrical housing.