Methods for producing enriched populations of human retinal pigment epithelium cells for treatment of retinal degeneration
First Claim
Patent Images
1. A method for the differentiation of hES cells into RPE cells, said method comprising:
- a) allowing hES cell cultures to overgrow on MEF and form a thick multilayer of cells, or forming an embryoid body (EB) from hES cells;
b) culturing the thick multilayer of cells or EB for a sufficient time for the appearance of pigmented cells comprising brown pigment dispersed in their cytoplasm; and
c) isolating and culturing the pigmented cells from the resultant cell cultures, thereby obtaining a culture comprising human RPE cells, wherein said human RPE cells are Pax6−
, bestrophin+, CRALBP+, PEDF+, express RPE65.
7 Assignments
0 Petitions
Accused Products
Abstract
This invention relates to methods for improved cell-based therapies for retinal degeneration and for differentiating human embryonic stem cells and human embryo-derived into retinal pigment epithelium (RPE) cells and other retinal progenitor cells.
-
Citations
42 Claims
-
1. A method for the differentiation of hES cells into RPE cells, said method comprising:
-
a) allowing hES cell cultures to overgrow on MEF and form a thick multilayer of cells, or forming an embryoid body (EB) from hES cells; b) culturing the thick multilayer of cells or EB for a sufficient time for the appearance of pigmented cells comprising brown pigment dispersed in their cytoplasm; and c) isolating and culturing the pigmented cells from the resultant cell cultures, thereby obtaining a culture comprising human RPE cells, wherein said human RPE cells are Pax6−
, bestrophin+, CRALBP+, PEDF+, express RPE65. - View Dependent Claims (2, 3, 4, 5, 6, 7)
-
-
8. A method of administering human RPE cells to an individual in need thereof, comprising:
-
a) allowing hES cell cultures to overgrow on MEF and form a thick multilayer of cells, or forming an embryoid body (EB) from hES cells; b) culturing the thick multilayer of cells or EB for a sufficient time for the appearance of pigmented cells comprising brown pigment dispersed in their cytoplasm; c) isolating and culturing the pigmented cells from the resultant cell cultures, thereby obtaining a culture comprising human RPE cells, wherein said human RPE cells are Pax6−
, bestrophin+, CRALBP+, PEDF+, and express RPE65; andd) introducing the human RPE cells into the individual in need thereof. - View Dependent Claims (9, 10, 11, 12, 13, 14)
-
-
15. A method for the treatment of Parkinson'"'"'s disease in an individual in need thereof, comprising:
-
a) allowing hES cell cultures to overgrow on MEF and form a thick multilayer of cells, or forming an embryoid body (EB) from hES cells; b) culturing the thick multilayer of cells or EB for a sufficient time for the appearance of pigmented cells comprising brown pigment dispersed in their cytoplasm; c) isolating and culturing the pigmented cells from the resultant cell cultures, thereby obtaining a culture comprising human RPE cells, wherein said human RPE cells are Pax6−
, bestrophin+, CRALBP+, PEDF+, and express RPE65; andd) introducing the human RPE cells into the individual in need thereof.
-
-
16. A method for the differentiation of hES cells into RPE cells, said method comprising:
-
a) allowing hES cell cultures to overgrow and form a thick multilayer of cells; b) culturing the thick multilayer of cells for a sufficient time for the appearance of pigmented cells comprising brown pigment dispersed in their cytoplasm; and c) isolating and culturing the pigmented cells from the resultant cell cultures, thereby obtaining a culture comprising human RPE cells, wherein said human RPE cells are Pax6−
, bestrophin+, CRALBP+, PEDF+, express RPE65. - View Dependent Claims (17, 18, 19, 20, 21, 22)
-
-
23. A method of administering human RPE cells to an individual in need thereof, comprising:
-
a) allowing hES cell cultures to overgrow and form a thick multilayer of cells; b) culturing the thick multilayer of cells for a sufficient time for the appearance of pigmented cells comprising brown pigment dispersed in their cytoplasm; c) isolating and culturing the pigmented cells from the resultant cell cultures, thereby obtaining a culture comprising human RPE cells, wherein said human RPE cells are Pax6−
, bestrophin+, CRALBP+, PEDF+, and express RPE65; andd) introducing the human RPE cells into the individual in need thereof. - View Dependent Claims (24, 25, 26)
-
-
27. A method for the treatment of Parkinson'"'"'s disease in an individual in need thereof, comprising:
-
a) allowing hES cell cultures to overgrow and form a thick multilayer of cells; b) culturing the thick multilayer of cells for a sufficient time for the appearance of pigmented cells comprising brown pigment dispersed in their cytoplasm; c) isolating and culturing the pigmented cells from the resultant cell cultures, thereby obtaining a culture comprising human RPE cells, wherein said human RPE cells are Pax6−
, bestrophin+, CRALBP+, PEDF+, and express RPE65; andd) introducing the human RPE cells into the individual in need thereof.
-
-
28. A method for the differentiation of hES cells into RPE cells, said method comprising:
-
a) forming an embryoid body (EB) from hES cells; b) culturing the EB for a sufficient time for the appearance of pigmented cells comprising brown pigment dispersed in their cytoplasm; and c) isolating and culturing the pigmented cells from the resultant cell cultures, thereby obtaining a culture comprising human RPE cells, wherein said human RPE cells are Pax6−
, bestrophin+, CRALBP+, PEDF+, express RPE65. - View Dependent Claims (29, 30, 31, 32, 33, 34, 35, 36, 37)
-
-
38. A method of administering human RPE cells to an individual in need thereof, comprising:
-
a) forming an embryoid body (EB) from hES cells; b) culturing the EB for a sufficient time for the appearance of pigmented cells comprising brown pigment dispersed in their cytoplasm; c) isolating and culturing the pigmented cells from the resultant cell cultures, thereby obtaining a culture comprising human RPE cells, wherein said human RPE cells are Pax6−
, bestrophin+, CRALBP+, PEDF+, and express RPE65; andd) introducing the human RPE cells into the individual in need thereof. - View Dependent Claims (39, 40, 41)
-
-
42. A method for the treatment of Parkinson'"'"'s disease in an individual in need thereof, comprising:
-
a) forming an embryoid body (EB) from hES cells; b) culturing the EB for a sufficient time for the appearance of pigmented cells comprising brown pigment dispersed in their cytoplasm; c) isolating and culturing the pigmented cells from the resultant cell cultures, thereby obtaining a culture comprising human RPE cells, wherein said human RPE cells are Pax6−
, bestrophin+, CRALBP+, PEDF+, and express RPE65; andd) introducing the human RPE cells into the individual in need thereof.
-
Specification