Methods, devices, kits and compositions for detecting roundworm

• US 8,268,574 B2
• Filed: 11/17/2010
• Issued: 09/18/2012
• Est. Priority Date: 05/19/2008
• Status: Active Grant

• Current Assignee: Monsanto Company (Bayer AG), IDEXX Laboratories Incorporated
• Original Assignee: Divergence, Inc. (Bayer AG), IDEXX Laboratories Incorporated
• Inventors: Elsemore, David Allen, Flynn, Laurie A., Crawford, Michael, Geng, Jinming
• Primary Examiner(s): BASKAR, PADMAVATHI
• Application Number:
  US12/948,503
• Publication Number:
  US 20110086340A1
• Time in Patent Office: 671 Days
• Field of Search: None
• US Class Current: 435/7.22
• CPC Class Codes: A61K 39/395 Antibodies agglutinins A61K38/36 ; as drug carriers A61K47/50; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
• CPC Class Codes: A61K 39/44 Antibodies bound to carriers
• CPC Class Codes: C07K 14/4354 from nematodes
• CPC Class Codes: C07K 16/00 Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies antibodies with enzymatic activity, e.g. abzymes C12N9/0002
• CPC Class Codes: C07K 16/18 against material from animals or humans
• CPC Class Codes: C12Q 1/6888 for detection or identification of organisms
• CPC Class Codes: G01N 33/5308 for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites

Abstract

Methods, devices, kits and compositions for detecting the presence or absence of roundworm in a mammalian sample are disclosed herein. The methods, devices, kits and compositions of the present invention may be used to confirm the presence or absence of roundworm in a fecal sample from a mammal that may also be infected with one or more of hookworm, whipworm, and heartworm. Confirmation of the presence or absence of roundworm in the mammal may be made, for example, for the purpose of selecting an optimal course of treating the mammal and/or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated.

Claims

1. A method of detecting the presence or absence of one or more roundworm antigens in a sample, the method comprising:

(a) contacting a sample from a mammal with one or more antibodies that specifically binds to polypeptide Copro6716, 10 kD isoform of excretory/secretory protein from T. canis;

(b) forming antibody antigen complexes in the presence of the roundworm antigens, if any, in the sample; and

(c) detecting the presence or absence of the antibody antigen complexes, if any.

2. The method of claim 1, wherein the sample is obtained from a mammal that is a canine or a feline.

3. The method of claim 2, wherein the roundworm is Toxocara canis or Toxocara cati.

4. The method of claim 1, wherein the sample is a fecal sample.

5. The method of claim 1, wherein the one or more antibodies do not specifically bind any coproantigen obtained from the group consisting of hookworm, whipworm, and heartworm.

6. The method of claim 1, wherein the step of detecting the presence or absence of the complexes further includes the step of providing at least one secondary antibody that binds to at least one of the complexes.

7. The method of claim 6, wherein the at least one secondary antibody is labeled.

8. The method of claim 1, wherein one or more of the one or more antibodies are labeled.

9. The method of claim 1, wherein the one or more antibodies are immobilized on a solid support.

10. The method of claim 9, wherein the solid support forms part of an enzyme-linked immunosorbent assay device.

11. The method of claim 10, wherein the enzyme-linked immunosorbent assay device is a lateral flow immunoassay device.

12. The method of claim 1, further comprising the step of contacting the sample with one or more reagents to detect one or more of the group consisting of:

one or more non-roundworm worm parasites, one or more non-worm parasites, one or more viruses, one or more fungi, and one or more bacteria.

13. The method of claim 12, wherein the reagents for the detection of any one or all of the one or more non-roundworm worm parasites, one or more non-worm parasites, one or more viruses, one or more fungi and one or more bacteria are one or more antibodies or one or more antigens recognized by antibodies specific for the one or more non-roundworm worm parasites, one or more non-worm parasites, one or more viruses, one or more fungi or one or more bacteria.

14. A method of diagnosing whether a mammal is infected with an intestinal roundworm infection, the method comprising the steps of:

(a) contacting a sample from a mammal with one or more antibodies that specifically bind to polypeptide Copro6716, 10 kD isoform of excretory/secretory protein from T. canis;

(b) forming antibody-antigen complexes in the presence of the roundworm antigens, if any, in the sample;

(c) detecting the presence or absence of the antibody-polypeptide complexes, if any; and

(d) diagnosing the mammal as having the roundworm infection if a roundworm antibody-antigen complex is present.

15. The method of claim 14, wherein the mammal is a canine or a feline.

16. The method of claim 14, wherein the roundworm is Toxocara canis or Toxocara cati.

17. The method of claim 14, wherein the step of detecting the presence or absence of the antibody-polypeptide complexes further includes the step of providing at least one secondary antibody that binds to the antibody-polypeptide complexes.

18. The method of claim 17, wherein the at least one secondary antibody is labeled.

19. The method of claim 14, wherein one or more of the one or more antibodies are labeled.

20. The method of claim 14, wherein the one or more antibodies are immobilized on a solid support.

21. The method of claim 20, wherein the solid support forms part of an enzyme-linked immunosorbent assay device.

22. The method of claim 21, wherein the enzyme-linked immunosorbent assay device is a lateral flow immunoassay device.

23. The method of claim 14, further comprising the step of contacting the sample with one or more reagents to detect one or more of the group consisting of:

one or more non-roundworm worm parasites, one or more non-worm parasites, one or more viruses, one or more fungi, and one or more bacteria.

24. The method of claim 14, wherein the sample is a fecal sample.

25. The method of claim 14, wherein the one or more antibodies do not specifically bind any coproantigen obtained from the group consisting of hookworm, whipworm, and heartworm.

26. The method of claim 25, wherein the reagents for the detection of any one or all of the one or more non-roundworm worm parasites, one or more non-worm parasites, one or more viruses, one or more fungi and one or more bacteria are one or more antibodies or one or more antigens recognized by antibodies specific for the one or more non-roundworm worm parasites, one or more non-worm parasites, one or more viruses, one or more fungi or one or more bacteria.