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Compositions and methods utilizing DNA polymerases

  • US 8,268,605 B2
  • Filed: 06/29/2001
  • Issued: 09/18/2012
  • Est. Priority Date: 10/29/1999
  • Status: Expired due to Term
First Claim
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1. A composition for identifying a nucleotide at a given position of a template DNA molecule, said composition comprising:

  • a Family B DNA polymerase deficient in 3′

    to 5′

    exonuclease activity comprising the amino acid sequence of one of SEQ ID NOs;

    2 and 62-78, excepta first mutation at amino acid D or E or both in the exo I (DXE) motif within said SEQ ID NO, anda second mutation at an amino acid selected from the group consisting of;

    amino acid L, P, and both L and P in Region II (DXXSLYPSII, SEQ ID NO;

    7) within said SEQ ID NO, wherein said second mutation confers a reduction in discrimination.

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