Molded cartridge with 3-D hydrodynamic focusing

• US 8,273,294 B2
• Filed: 06/30/2006
• Issued: 09/25/2012
• Est. Priority Date: 07/01/2005
• Status: Active Grant

• Current Assignee: Honeywell International Inc.
• Original Assignee: Honeywell International Inc.
• Inventors: Padmanabhan, Aravind, Bardell, Ron L.
• Primary Examiner(s): Turk, Neil N
• Application Number:
  US11/428,370
• Publication Number:
  US 20070009386A1
• Time in Patent Office: 2,279 Days
• Field of Search: None
• US Class Current: 422/63
• CPC Class Codes: B01L 2200/0621 Control of the sequence of chambers filled or emptied
• CPC Class Codes: B01L 2200/0636 Focussing flows, e.g. to laminate flows
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2300/0877 Flow chambers
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502776 specially adapted for focusing or laminating flows
• CPC Class Codes: G01N 15/1404 Handling flow, e.g. hydrodynamic focusing
• CPC Class Codes: G01N 15/1484 microstructural devices
• CPC Class Codes: G01N 2015/016 White blood cells
• CPC Class Codes: G01N 2015/1486 Counting the particles
• CPC Class Codes: Y10T 436/117497 with a continuously flowing sample or carrier stream
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A microfluidic circuit cartridge having 3-D hydrodynamic focusing. The cartridge may be fabricated with injection-molded or other molded layers providing a 3-D structure. A flow channel on the card may have a sample core flowing in a fluid of a flow channel for analysis. The sample core may be adjustable in position within the channel with one or more jets or channels of fluid being injected into the flow channel. The jets may also adjust the size of the sample core. There may be a hemoglobin measurement mechanism or card with a cuvette.

Claims

1. A molded fluidic circuit card comprising:

a first channel having a longitudinal axis and a first fluid flow;

a second channel situated in the first channel, the second channel introducing a sample core flow within the first fluid flow;

a third channel coupled to the first channel and having a longitudinal axis at an angle relative to the longitudinal axis of the first channel;

a fourth channel coupled to the first channel and having a longitudinal axis at an angle relative to the longitudinal axis of the first channel, the fourth channel positioned at a location relative to the third channel to achieve three-dimensional focusing of the core sample;

a first fluid reservoir coupled to the third channel;

a second fluid reservoir coupled to the fourth channel;

a flow rate control box in communication with the third and fourth channels, the flow rate control box controlling the flow of a second fluid from the first fluid reservoir through the third channel and into and through the first channel and the flow rate control box controlling the flow of a second fluid from the second fluid reservoir through the fourth channel and into and through the first channel;

wherein the flow rate control box controls a velocity of fluid flow in the third channel relative to a velocity of fluid flow in the fourth channel to adjust the sample core flow in three dimensions, thereby achieving three-dimensional focusing of the sample core flow;

wherein the first, second, third, and fourth channels and first and second fluid reservoirs are molded components in the fluidic circuit card.

2. The card of claim 1, wherein the fluidic circuit card comprises a cytometer.

3. The card of claim 1, wherein a position of the sample core flow is adjustable by changing a ratio of a velocity of the fluid from the third channel and a velocity of the fluid from the fourth channel.

4. The card of claim 1, wherein a size of the sample core flow is adjustable by changing a ratio of a velocity of the sample core flow and a velocity of the fluid from the third channel and the fourth channel.

5. A molded microfluidic analyzer card comprising:

a flow channel;

a sample core flow situated in the flow channel;

a plurality of control channels each having a longitudinal axis coupled to the flow channel configured to introduce a steering fluid into the flow channel to adjust the sample core flow;

a flow rate control box configured to control fluid flow in the plurality of control channels;

wherein each of the plurality of control channels is positioned at an angle to the longitudinal axes of the remaining plurality of control channels;

wherein the flow rate control box adjusts a position of the sample core flow by changing a ratio of the velocities of the steering fluids entering the flow channel from a first and a second control channel relative to each other;

wherein the flow rate control box adjusts a size of the sample core flow by changing a ratio of the velocities of the steering fluids entering the flow channel from a first and a second control channel relative to each other;

wherein the flow channel and the plurality of control channels are molded components in the microfluidic card.

6. The card of claim 5, wherein the flow channel is a cytometer flow channel.

7. The card of claim 5, wherein:

the flow channel includes a flow of a first fluid; and

the sample core flow is a sample core in the flow of the first fluid.

8. The card of claim 7, wherein the first fluid is a reagent.

9. A molded fluidic circuit card comprising:

a first channel having a longitudinal axis;

a second channel disposed in the first channel;

a first control channel coupled to the first channel and having a longitudinal axis at an angle relative to the longitudinal axis of the first channel; and

a second control channel coupled to the first channel and having a longitudinal axis at an angle relative to the longitudinal axis of the first channel, the second control channel positioned at an angle relative to the longitudinal axis of the first control channel; and

a flow rate control box in communication with the first control channel and the second control channel; and

wherein;

the first channel includes a first fluid flow;

the second channel introduces a sample core flow within the first fluid flow;

the first control channel is coupled to a first fluid reservoir and the second control channel is coupled to a second fluid reservoir such that a second fluid may flow from the first and second fluid reservoirs through the first and second control channels into the first channel in a single direction to adjust the sample core flow; and

the flow rate control box controls a velocity of fluid flow in the first control channel relative to a velocity of fluid flow in the second control channel to adjust the sample core flow in three dimensions, thereby achieving three-dimensional focusing of the sample core flow;

wherein the first and second channels, first and second control channels, and first and second fluid reservoirs are molded components in the fluidic circuit card.

10. The card of claim 9, wherein a position of the sample core flow is adjustable by changing a ratio of a velocity of the fluid from the first control channel and a velocity of the fluid from the second control channel.

11. The card of claim 9, wherein a size of the sample core flow is adjustable by changing a ratio of a velocity of the sample core flow and a velocity of the fluid from the first control channel and the second control channel.