Method of isolating stem and progenitor cells from placenta
First Claim
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1. A method of obtaining fetal stem and progenitor cells from a cryopreserved mammalian placenta, the method comprising:
- a) perfusing a cryopreserved mammalian placenta with a collection solution comprising an effective amount of an agent that induces mobilization of a stem and/or progenitor cell into a blood vessel of the placenta, wherein the mobilization agent is AMD-3100, a biocompatible non-ionic surfactant, an antibody that binds specifically to an adhesion molecule and that inhibits cell adhesion, a thrombolytic enzyme, or ethylenediamine tetraacetic acid; and
b) collecting the mobilized cells from the blood vessel, wherein the collected cells comprise fetal stem and progenitor cells.
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Abstract
The present invention provides methods of cryopreserving stem and progenitor cells in a mammalian placenta; and methods of obtaining fetal stem and progenitor cells from a cryopreserved mammalian placenta. Cells obtained by carrying out the methods can be used in a variety of therapeutic applications.
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Citations
18 Claims
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1. A method of obtaining fetal stem and progenitor cells from a cryopreserved mammalian placenta, the method comprising:
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a) perfusing a cryopreserved mammalian placenta with a collection solution comprising an effective amount of an agent that induces mobilization of a stem and/or progenitor cell into a blood vessel of the placenta, wherein the mobilization agent is AMD-3100, a biocompatible non-ionic surfactant, an antibody that binds specifically to an adhesion molecule and that inhibits cell adhesion, a thrombolytic enzyme, or ethylenediamine tetraacetic acid; and b) collecting the mobilized cells from the blood vessel, wherein the collected cells comprise fetal stem and progenitor cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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