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Process of inducing differentiation of embryonic cell to cell expressing neural surface marker using OP9 or PA6 cells

  • US 8,273,570 B2
  • Filed: 05/16/2001
  • Issued: 09/25/2012
  • Est. Priority Date: 05/16/2000
  • Status: Expired due to Fees
First Claim
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1. A method for producing a cell expressing a neural crest marker or a neural tube marker, comprising:

  • culturing under serum-free conditions a mammalian embryonic stem cell in vitro in the absence of retinoic acid and in the presence of a stroma cell without forming embryoid body, wherein the stroma cell is OP9 cell or PA6 cell.

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