Methods and apparatus for centrifugal liquid chromatography
First Claim
1. A chromatographic system comprising:
- a chromatographic enclosure said chromatographic enclosure being arranged to contain an associated chromatographic stationary phase and to facilitate transmission of a fluid through the chromatographic stationary phase contained within the chromatographic enclosure;
a rotor that carries the chromatographic enclosure wherein the rotor is configured to rotate the chromatographic enclosure at an angular velocity such that fluid is driven through chromatographic enclosure including through the chromatographic stationary phase via centrifugal force; and
a fluid enclosure including a first portion configured to remain stationary while the rotor is rotating and a second portion, carried on the rotor, configured to rotate with the rotor wherein the fluid enclosure is in fluid communication with the chromatographic enclosure, and wherein the fluid enclosure is a mobile phase fluid reservoir arranged to hold a mobile phase fluid intended for delivery to the chromatographic enclosure on the rotor.
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Abstract
Apparatus and methods related to centrifugal liquid chromatography are described. An angular velocity can be simultaneously imparted to a large number of chromatographic enclosures. Via centrifugal forces, a mobile phase fluid including a sample can be driven through a stationary phase within the chromatographic enclosure to perform a chromatographic separation process on components of the sample. The use of centrifugation as a driving force can allow significantly smaller stationary phase particles to be employed as compared to high performance liquid chromatography (HPLC). Further, for an equivalent chromatographic separation process, the use of centrifugation can provide much greater separation efficiencies than HPLC.
279 Citations
23 Claims
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1. A chromatographic system comprising:
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a chromatographic enclosure said chromatographic enclosure being arranged to contain an associated chromatographic stationary phase and to facilitate transmission of a fluid through the chromatographic stationary phase contained within the chromatographic enclosure; a rotor that carries the chromatographic enclosure wherein the rotor is configured to rotate the chromatographic enclosure at an angular velocity such that fluid is driven through chromatographic enclosure including through the chromatographic stationary phase via centrifugal force; and a fluid enclosure including a first portion configured to remain stationary while the rotor is rotating and a second portion, carried on the rotor, configured to rotate with the rotor wherein the fluid enclosure is in fluid communication with the chromatographic enclosure, and wherein the fluid enclosure is a mobile phase fluid reservoir arranged to hold a mobile phase fluid intended for delivery to the chromatographic enclosure on the rotor. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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21. A centrifugal chromatographic system comprising:
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a rotor that carries at least one chromatographic enclosure wherein the rotor is configured to rotate the chromatographic enclosure such that fluid is driven through at least one chromatographic enclosure by centrifugal force; and a fluid delivery mechanism in fluid communication with the chromatographic enclosure to facilitate the delivery of a mobile phase fluid to the chromatographic enclosure, wherein the fluid delivery mechanism includes a first portion configured to remain stationary while the rotor is rotating and a second portion carried on the rotor and configured to rotate with the rotor, the fluid delivery mechanism being arranged so that fluid can be passed from the first portion to the second portion of the fluid delivery mechanism while the rotor is rotating, and wherein the fluid delivery mechanism includes a mobile phase fluid reservoir arranged to hold a mobile phase fluid intended for delivery to the chromatographic enclosure on the rotor. - View Dependent Claims (22, 23)
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Specification