Methods and apparatus for centrifugal liquid chromatography
First Claim
1. A chromatographic system comprising:
- a rotor configured to rotate about an axis, said rotor comprising;
an enclosed flow path configured to contain a fluid under pressure including
1) a first segment for receiving the fluid from an entrance to the flow path and communicating the fluid away from the axis and
2) a second segment in fluid communication with the first segment for communicating the fluid toward the axis and out an exit to the flow path, said exit located at a first radial distance from the axis, such that during a rotation of the rotor the fluid continually drains from the exit when a fluid level in the first segment is located at a second radial distance from the axis less than the first radial distance; and
a chromatographic enclosure, carried on the rotor, located between the entrance and the exit of the fluid conduit, said chromatographic enclosure being arranged to contain an associated chromatographic stationary phase and to facilitate transmission of a fluid through the chromatographic stationary phase contained within the chromatographic enclosure, wherein the fluid is driven through the chromatographic stationary phase via centrifugal force generated from the rotation of the rotor.
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Abstract
Apparatus and methods related to centrifugal liquid chromatography are described. An angular velocity can be simultaneously imparted to a large number of chromatographic enclosures. Via centrifugal forces, a mobile phase fluid including a sample can be driven through a stationary phase within the chromatographic enclosure to perform a chromatographic separation process on components of the sample. The use of centrifugation as a driving force can allow significantly smaller stationary phase particles to be employed as compared to high performance liquid chromatography (HPLC). Further, for an equivalent chromatographic separation process, the use of centrifugation can provide much greater separation efficiencies than HPLC.
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Citations
29 Claims
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1. A chromatographic system comprising:
a rotor configured to rotate about an axis, said rotor comprising; an enclosed flow path configured to contain a fluid under pressure including
1) a first segment for receiving the fluid from an entrance to the flow path and communicating the fluid away from the axis and
2) a second segment in fluid communication with the first segment for communicating the fluid toward the axis and out an exit to the flow path, said exit located at a first radial distance from the axis, such that during a rotation of the rotor the fluid continually drains from the exit when a fluid level in the first segment is located at a second radial distance from the axis less than the first radial distance; anda chromatographic enclosure, carried on the rotor, located between the entrance and the exit of the fluid conduit, said chromatographic enclosure being arranged to contain an associated chromatographic stationary phase and to facilitate transmission of a fluid through the chromatographic stationary phase contained within the chromatographic enclosure, wherein the fluid is driven through the chromatographic stationary phase via centrifugal force generated from the rotation of the rotor. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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22. A chromatographic system comprising:
a rotor configured to rotate about an axis, said rotor comprising; a fluid conduit, having an internal area, an entrance and an exit located at a first radial distance from the axis, including
1) a first segment for receiving a fluid from the entrance and communicating the fluid away from the axis and
2) a second segment in fluid communication with the first segment for communicating the fluid toward the axis and out the exit such that during a rotation of the rotor the fluid continually drains from the exit when a fluid level in the first segment is at a second radial distance less than the first radial distance of the exit;a chromatographic enclosure, carried on the rotor, located between the entrance and the exit of the fluid conduit, said chromatographic enclosure being arranged to contain an associated chromatographic stationary phase and to facilitate transmission of a fluid through the chromatographic stationary phase contained within the chromatographic enclosure, wherein the fluid is driven through the chromatographic stationary phase via centrifugal force generated from the rotation of the rotor. - View Dependent Claims (23, 24, 25, 26, 27, 28, 29)
Specification