Microfluidic flow cell
First Claim
1. A microfluidic flow cell, comprising:
- a body having a fluid transport channel disposed therein, the fluid transport channel having a proximal end and a distal end defining a fluid flow path;
a fluid inlet port disposed at the proximal end of the fluid transport channel at a central portion of the body and an outlet port disposed at the distal end of the fluid transport channel at an outer portion of the body; and
a plurality of sample wells in direct fluid communication with the fluid transport channel and substantially perpendicular to the fluid flow path in the fluid transport channel such that a volume of a liquid medium flowing through the fluid transport channel will flow into at least a first sample well with an excess of the liquid medium flowing into at least a second sample well.
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Accused Products
Abstract
A microfluidic flow cell having a body with a fluid transport channel disposed therein, the fluid transport channel having a proximal end and a distal end defining a fluid flow path, a fluid inlet port disposed at the proximal end of the fluid transport channel at a central portion of the body and an outlet port disposed at the distal end of the fluid transport channel at an outer portion of the body, and a plurality sample wells disposed in the fluid transport channel substantially perpendicular to the fluid flow path in the fluid transport channel. The microfluidic flow cell may have hundreds or thousands of individual, sub-microliter sample wells. The microfluidic flow cell can be filled by applying a flowable liquid to the inlet port and spinning the flow cell to cause fluid to flow into fluid transport channel. The microfluidic flow cells described herein can be used in a variety of applications where small sample size and/or a large number of replicates are desirable.
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Citations
47 Claims
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1. A microfluidic flow cell, comprising:
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a body having a fluid transport channel disposed therein, the fluid transport channel having a proximal end and a distal end defining a fluid flow path; a fluid inlet port disposed at the proximal end of the fluid transport channel at a central portion of the body and an outlet port disposed at the distal end of the fluid transport channel at an outer portion of the body; and a plurality of sample wells in direct fluid communication with the fluid transport channel and substantially perpendicular to the fluid flow path in the fluid transport channel such that a volume of a liquid medium flowing through the fluid transport channel will flow into at least a first sample well with an excess of the liquid medium flowing into at least a second sample well. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 17, 18, 19, 20, 21, 22, 23, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47)
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10. A microfluidic flow cell, comprising:
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a disc-shaped body configured for spinning about a central axis, the body further including; at least one fluid inlet port situated toward a center portion of the disc and at least one fluid outlet port situated toward an outer edge of the disc; at least one fluid transport channel defining a fluid flow path in the body, the fluid transport channel being disposed in the body such that it is in fluid communication with a fluid inlet port and a fluid outlet port; and a plurality of sample wells in direct fluid communication with the at least one fluid transport channel such that the sample wells are substantially perpendicular to the fluid flow path, wherein a fluid applied at the inlet port will flow through the body along the fluid path toward the outlet port in response to spinning the disc-shaped body about the central axis. - View Dependent Claims (11, 12, 13, 14, 15, 16)
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24. A method for manufacturing a microfluidic flow cell, comprising:
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providing a fluid transport layer, comprising; forming a fluid transport channel defining a fluid flow path in the fluid transport layer, the fluid transport channel having a proximal end at a central portion of the fluid transport layer and a distal end at an outer portion of the fluid transport layer; forming a plurality sample wells in direct fluid communication with the fluid transport channel, the plurality sample wells being substantially perpendicular to the fluid flow path in the fluid transport channel; and
sealing the fluid transport layer with at least one outer layer. - View Dependent Claims (25, 26, 27, 28, 29, 30, 31, 32)
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Specification