Unitary cartridge for particle processing

• US 8,277,764 B2
• Filed: 12/05/2005
• Issued: 10/02/2012
• Est. Priority Date: 12/03/2004
• Status: Active Grant

• Current Assignee: Cytonome/St, Llc (Cytonome Inc.)
• Original Assignee: Cytonome/St, Llc (Cytonome Inc.)
• Inventors: Gilbert, John R., Lewis, Hugh, Beaupre, Derek, Trikha, Jaishree, Deshpande, Manish
• Primary Examiner(s): Nagpaul, Jyoti
• Application Number:
  US11/295,183
• Publication Number:
  US 20060269446A1
• Time in Patent Office: 2,493 Days
• Field of Search: 422 99-102, 422/50, 422500-502, 436/43, 436 65- 67, 436/180, 435/287.2
• US Class Current: 422/554
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/0636 Focussing flows, e.g. to laminate flows
• CPC Class Codes: B01L 2200/0647 Handling flowable solids, e.g. microscopic beads, cells, particles
• CPC Class Codes: B01L 2200/082 Handling hazardous material
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2300/0809 rectangular shaped
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: B01L 3/502776 specially adapted for focusing or laminating flows
• CPC Class Codes: B01L 5/00 Gas handling apparatus gas jars or cylinders B01L3/12; cold traps or cold baffles B01D8/00
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: C12M 47/04 Cell isolation or sorting purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04
• CPC Class Codes: G01N 15/1023 Microstructural devices for non-optical measurement
• CPC Class Codes: G01N 15/1484 microstructural devices
• CPC Class Codes: G01N 15/149 specially adapted for sorting particles, e.g. by their size or optical properties
• CPC Class Codes: G01N 2015/1486 Counting the particles
• CPC Class Codes: G01N 33/577 involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens; G01N33/53 - G01N33/576 take precedence
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A single disposable cartridge for performing a process on a particle, such as particle sorting, encapsulates all fluid contact surfaces in the cartridge for use with microfluidic particle processing technology. The cartridge interfaces with an operating system for effecting particle processing. The encapsulation of the fluid contact surfaces insures, improves or promotes operator isolation and/or product isolation. The cartridge may employ any suitable technique for processing particles.

Claims

1. A unitary particle processing cartridge for performing a process on a sample, the unitary particle processing cartridge comprising:

a microfluidic particle processing component for processing a sample, the microfluidic particle processing component including an input for receiving the sample, a processing region for processing the sample and one or more outputs for outputting the processed sample, the microfluidic particle processing component having at least one surface exposed to an exterior environment external to the unitary particle processing cartridge;

one or more fluid chambers external to the microfluidic particle processing component and in fluid communication with each of the input and the one or more outputs of the microfluidic particle processing component, each of the fluid chambers including one or more fluid contact surfaces, wherein the fluid contact surfaces are encapsulated and sealed in the unitary particle processing cartridge from the exterior environment; and

a sealable sample extraction port in fluid communication with at least one of the one or more fluid chambers and with the exterior environment for extracting a processed sample from the unitary particle processing cartridge.

2. The unitary particle processing cartridge of claim 1, further comprising a sealable sample input port in fluid communication with at least one of the fluid chambers for inputting a sample to be processed into the unitary particle processing cartridge.

3. The unitary particle processing cartridge of claim 1, wherein at least one of the fluid chambers comprises a sample input chamber for storing a sample prior to processing.

4. The unitary particle processing cartridge of claim 1, wherein at least one of the fluid chambers comprises a processed sample chamber for storing a sample after processing.

5. The unitary particle processing cartridge of claim 4, further comprising a filter disposed downstream of the processed sample chamber to remove supernatant from the processed sample chamber.

6. The unitary particle processing cartridge of claim 5, further comprising a trap filter for removing microbes from the removed supernatant.

7. The unitary particle processing cartridge of claim 6, wherein the trap filter is removable from the cartridge to allow for microbial testing of the supernatant.

8. The unitary particle processing cartridge of claim 1, wherein the microfluidic particle processing component comprises a processing means in the processing region for processing the sample.

9. The unitary particle processing cartridge of claim 1, wherein the microfluidic particle processing component comprises a particle sorting component disposed in the processing region for sorting particles in a sample having a predetermined characteristic from particles in the sample that do not have the predetermined characteristic.

10. The unitary particle processing cartridge of claim 1, wherein the microfluidic particle processing component comprises a particle concentration component for increasing a concentration of a particle in the sample having a predetermined characteristic and a particle sorting component disposed in the processing region for sorting particles in the sample having the predetermined characteristic from particles in the sample that do not have the predetermined characteristic.

11. The unitary particle processing cartridge of claim 1, wherein the unitary particle processing cartridge includes two or more chambers rigidly linked.

12. The unitary particle processing cartridge of claim 1, wherein the microfluidic particle processing component comprises a mixing component for mixing a particle in the sample having a predetermined characteristic with a bead or reagent.

13. The unitary particle processing cartridge of claim 1, wherein the microfluidic particle processing component is capable of performing a microfluidic process on the sample.

14. The unitary particle processing cartridge of claim 1, wherein the microfluidic particle processing component is capable of performing an electrical, a mechanical, a pneumatic, an optical or a magnetic process on the sample.

15. The unitary particle processing cartridge of claim 1, wherein the unitary particle processing cartridge includes a cartridge substrate defining the one or more chambers.

16. The unitary particle processing cartridge of claim 15, wherein the microfluidic particle processing component is integrally formed relative to the cartridge substrate.

17. The unitary particle processing cartridge of claim 15, wherein the microfluidic particle processing component is secured relative to the cartridge substrate.

18. The unitary particle processing cartridge of claim 15, wherein the microfluidic particle processing component is separately inserted into and removable from the cartridge substrate.

19. The unitary particle processing cartridge of claim 1, wherein the microfluidic particle processing component is interchangeable, and wherein the microfluidic particle processing component is selected based on the process to be performed on the sample.

20. The unitary particle processing cartridge of claim 1, wherein the unitary particle processing cartridge comprises a plurality of microfluidic particle processing components.

21. The unitary particle processing cartridge of claim 1, wherein the microfluidic particle processing component is a microfluidic sorting chip.

22. The unitary particle processing cartridge of claim 1, wherein the cartridge includes a reservoir tray and a reservoir cover.

23. The unitary particle processing cartridge of claim 1, wherein the unitary particle processing cartridge is adapted for modular association with an operating system.

24. The unitary particle processing cartridge of claim 23, wherein the operating system provides actuating means for the microfluidic particle processing component for the processing of the sample.

25. The unitary particle processing cartridge of claim 23, wherein the unitary particle processing cartridge is an interchangeable and disposable component of the operating system.

26. A unitary particle processing cartridge for performing a process on a sample, the unitary particle processing cartridge comprising:

one or more fluid chambers;

including one or more fluid contact surfaces encapsulated and sealed in the unitary particle processing cartridge from an exterior environment;

a sealable sample extraction port in fluid communication with at least one of the one or more fluid chambers and with the exterior environment for extracting a processed sample from the unitary particle processing cartridge; and

a holder for receiving a microfluidic particle processing component, whereby at least one surface of the microfluidic particle processing component is exposed to the exterior environment;

wherein the unitary particle processing cartridge is adapted to cooperate with the microfluidic particle processing component, whereby at least one of the one or more fluid chambers is placed in fluid communication with the microfluidic particle processing component.

27. The unitary particle processing cartridge of claim 26, wherein the microfluidic particle processing component is integrally formed relative to the unitary particle processing cartridge.

28. The unitary particle processing cartridge of claim 26, wherein the microfluidic particle processing component is separately formed relative to the unitary particle processing cartridge.

29. The unitary particle processing cartridge of claim 26, wherein the microfluidic particle processing component is removably securable relative to the unitary particle processing cartridge.

30. The unitary particle processing cartridge of claim 26, wherein the microfluidic particle processing component is interchangeable, and wherein the microfluidic particle processing component is selected based on the process to be performed on the sample.

31. The unitary particle processing cartridge of claim 26, wherein the unitary particle processing cartridge is adapted to cooperate with a plurality of microfluidic particle processing components.

32. The unitary particle processing cartridge of claim 26, wherein the microfluidic particle processing component is a microfluidic sorting chip.

33. The unitary particle processing cartridge of claim 26, wherein the unitary particle processing cartridge includes a reservoir tray and a reservoir cover.

34. The unitary particle processing cartridge of claim 26, wherein the unitary particle processing cartridge is adapted for modular association with an operating system.

35. The unitary particle processing cartridge of claim 34, wherein the operating system. provides actuating means for the microfluidic particle processing component for the processing of the sample.

36. The unitary particle processing cartridge of claim 34, wherein the unitary particle processing cartridge is an interchangeable and disposable component of the operating system.

37. The unitary particle processing cartridge of claim 26, further comprising a sealable sample input port in fluid communication with at least one of the fluid chambers for inputting a sample to be processed into the unitary particle processing cartridge.

38. The unitary particle processing cartridge of claim 26, wherein at least one of the fluid chambers is a sample input chamber for storing a sample prior to processing.

39. The unitary particle processing cartridge of claim 26, wherein at least one of the fluid chambers is a processed sample chamber for storing a sample after processing.

40. The unitary particle processing cartridge of claim 39, further comprising a filter disposed downstream of the processed sample chamber to remove supernatant from the processed sample chamber.

41. The unitary particle processing cartridge of claim 40, further comprising a recycling conduit for recycling the removed supernatant back to a sheath fluid reservoir that is adapted to provide sheath fluid to the microfluidic particle processing component.

42. The unitary particle processing cartridge of claim 40, further comprising a trap filter for removing microbes from the removed supernatant.

43. The unitary particle processing cartridge of claim 42, wherein the trap filter is removable from the cartridge to allow for microbial testing of the supernatant.

44. The unitary particle processing cartridge of claim 26, wherein the unitary particle processing cartridge includes two or more chambers rigidly linked.

45. A unitary particle processing cartridge for performing a process on a sample, the unitary particle processing cartridge comprising:

a microfluidic particle processing component for processing a sample, the microfluidic particle processing component including an input for receiving the sample, a processing region for processing the sample and one or more outputs for outputting the processed sample, the microfluidic particle processing component having at least one surface exposed to an exterior environment external to the unitary particle processing cartridge;

one or more fluid chambers external to the microfluidic particle processing component and in fluid communication with each of the input and the one or more outputs of the microfluidic particle processing component, each of the fluid chambers including one or more fluid contact surfaces, wherein the fluid contact surfaces are encapsulated and sealed in the unitary particle processing cartridge from the exterior environment, wherein at least one of the fluid chambers comprises a processed sample chamber for storing a sample after processing;

a sealable sample extraction port in fluid communication with at least one of the one or more fluid chambers and with the exterior environment for extracting a processed sample from the unitary particle processing cartridge;

a filter disposed downstream of the processed sample chamber to remove supernatant from the processed sample chamber.

46. A unitary particle processing cartridge for performing a process on a sample, the unitary particle processing cartridge comprising:

a microfluidic particle processing component for processing a sample, the microfluidic particle processing component including an input for receiving the sample, a processing region for processing the sample and one or more outputs for outputting the processed sample, the microfluidic particle processing component having at least one surface exposed to an exterior environment external to the unitary particle processing cartridge;

one or more fluid chambers external to the microfluidic particle processing component and in fluid communication with each of the input and the one or more outputs of the microfluidic particle processing component, each of the fluid chambers including one or more fluid contact surfaces, wherein the fluid contact surfaces are encapsulated and sealed in the unitary particle processing cartridge from the exterior environment; and

a sealable sample extraction port in fluid communication with at least one of the one or more fluid chambers and with the exterior environment for extracting a processed sample from the unitary particle processing cartridge;

wherein the microfluidic particle processing component comprises a particle concentration component for increasing a concentration of a particle in the sample having a predetermined characteristic;

a recycling conduit for recycling the removed supernatant from the processed sample chamber back to a sheath fluid reservoir that provides sheath fluid to the microfluidic particle processing component.