Method for detecting a protease
First Claim
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1. A method for detecting a protease, comprising:
- contacting a sample to be assayed with a detection means comprising a substrate at least partially coated with a film of a synthetic polymeric matrix; and
measuring a signal output of said detection means;
wherein the synthetic polymeric matrix comprises a polymer which is oxidised dextran and wherein the synthetic polymeric matrix is cross-linked with a peptide of up to 20 amino acids or wherein a peptide of up to 20 amino acids is integrated into the backbone of the synthetic polymetric matrix, wherein the synthetic polymeric matrix degrades due to the action of the protease on the peptide.
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Abstract
Methods for detecting proteases by contacting a sample to be assayed with a substrate at least partially coated with a film of a synthetic polymeric matrix, and measuring a signal output of the substrate is provided herein.
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12 Claims
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1. A method for detecting a protease, comprising:
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contacting a sample to be assayed with a detection means comprising a substrate at least partially coated with a film of a synthetic polymeric matrix; and measuring a signal output of said detection means; wherein the synthetic polymeric matrix comprises a polymer which is oxidised dextran and wherein the synthetic polymeric matrix is cross-linked with a peptide of up to 20 amino acids or wherein a peptide of up to 20 amino acids is integrated into the backbone of the synthetic polymetric matrix, wherein the synthetic polymeric matrix degrades due to the action of the protease on the peptide. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 12)
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12. The method of claim 5 wherein the electrode is composed of noble metals or carbon.
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11. A method for detecting a protease comprising:
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contacting a sample to be assayed with a detection means comprising a substrate at least partially coated with a film of a synthetic polymeric matrix; and measuring a signal output of said detection means; wherein the synthetic polymeric matrix comprises a polymer and wherein the synthetic polymeric matrix is cross-linked with a peptide of up to 20 amino acids or wherein a peptide of up to 20 amino acids is integrated into the backbone of the synthetic polymeric matrix.
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Specification