Amnion-derived cell compositions, methods of making and uses thereof
First Claim
1. A substantially purified population of cultured amnion-derived epithelial cells that is made by the method comprising the steps ofa) obtaining a placenta and isolating an amnion from the placenta,b) enzymatically releasing amnion-derived epithelial cells from the amnion,c) collecting the released amnion-derived epithelial cells, andd) culturing the collected amnion-derived epithelial cells of step (c) in basal culture medium that is supplemented with human serum albumin and recombinant human EGF.
2 Assignments
0 Petitions
Accused Products
Abstract
The invention is directed to substantially purified amnion-derived cell populations, compositions comprising the substantially purified amnion-derived cell populations, and to methods of creating such substantially purified amnion-derived cell populations, as well as methods of use. The invention is further directed to antibodies, in particular, monoclonal antibodies, that bind to amnion-derived cells or, alternatively, to one or more amnion-derived cell surface protein markers. The invention is further directed to methods for producing the antibodies, methods for using the antibodies, and kits comprising the antibodies.
-
Citations
9 Claims
-
1. A substantially purified population of cultured amnion-derived epithelial cells that is made by the method comprising the steps of
a) obtaining a placenta and isolating an amnion from the placenta, b) enzymatically releasing amnion-derived epithelial cells from the amnion, c) collecting the released amnion-derived epithelial cells, and d) culturing the collected amnion-derived epithelial cells of step (c) in basal culture medium that is supplemented with human serum albumin and recombinant human EGF.
Specification