×

Asymmetric adapter library construction

  • US 8,288,097 B2
  • Filed: 09/01/2011
  • Issued: 10/16/2012
  • Est. Priority Date: 04/30/2008
  • Status: Active Grant
First Claim
Patent Images

1. A method of producing an asymmetrically tagged nucleic acid fragment, said method comprising:

  • i) ligating an adapter to each end of a double-stranded nucleic acid fragment, wherein said adapter comprises;

    (a) a first and a second nucleic acid strand associated with each other via one or more complementary domains, said adapter having a first end and a second end;

    (b) one or more region of substantial non-complementarity between said first and second nucleic acid strands;

    (c) a ligation site positioned on said first end of said adapter configured to allow ligation of said adapter to said double stranded nucleic acid fragment; and

    (d) a hairpin structure positioned on the 3′

    strand on said second end of said adaptor, said hairpin structure producing a nucleic acid synthesis self-priming site; and

    ii) performing a first round of nucleic acid synthesis initiated from said self-priming site using a nucleotide polymerase, thereby producing an asymmetrically tagged nucleic acid fragment.

View all claims
  • 3 Assignments
Timeline View
Assignment View
    ×
    ×