Bioreactive agents
First Claim
1. A method, comprising, a) providing a conjugate comprising a bioreactive agent bound to a substrate, wherein said substrate comprises a nucleotide, said bioreactive agent comprises a cleavable moiety and a detectable moiety, wherein said cleavable moiety is positioned between said detectable moiety and said substrate;
- b) contacting said conjugate with a mixture to create a coupled conjugate;
c) removing uncoupled conjugates; and
d) treating said coupled conjugate so as to release said detectable moiety.
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Accused Products
Abstract
This invention relates to agents and conjugates to detect and isolate target components from complex mixtures such as nucleic acids from biological samples, cells from bodily fluids, and nascent proteins from translation reactions. Agents comprise a detectable moiety bound to a photoreactive moiety. Conjugates comprise agents coupled to substrates by covalent bounds which can be selectively cleaved with the administration of electromagnetic radiation. Targets substances labeled with detectable molecules can be easily identified and separated from a heterologous mixture of substances. Exposure of the conjugate to radiation releases the target in a functional form and completely unaltered. Using photocleavable molecular precursors as the conjugates, label can be incorporated into macromolecules, the nascent macromolecules isolated and the label completely removed. The invention also relates to targets isolated with these conjugates which may be useful as pharmaceutical agents or compositions that can be administered to humans and other mammals.
7 Citations
14 Claims
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1. A method, comprising, a) providing a conjugate comprising a bioreactive agent bound to a substrate, wherein said substrate comprises a nucleotide, said bioreactive agent comprises a cleavable moiety and a detectable moiety, wherein said cleavable moiety is positioned between said detectable moiety and said substrate;
- b) contacting said conjugate with a mixture to create a coupled conjugate;
c) removing uncoupled conjugates; and
d) treating said coupled conjugate so as to release said detectable moiety. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
- b) contacting said conjugate with a mixture to create a coupled conjugate;
Specification