Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids
First Claim
1. A process for culturing a microorganism of the order Thraustochytriales for the production of lipids, the process comprising:
- culturing the microorganism of the order Thraustochytriales in a culture medium that comprises a chloride concentration of less than about 500 mg/L and a non-chloride source of sodium selected from the group consisting of soda ash, sodium carbonate, sodium bicarbonate, sodium sulfate and mixtures thereof, and wherein the microorganism after culturing has a sterol content of at least about 0.1% ash-free dry weight (afdw) and a cholesterol content of at least about 15% of the total sterol content.
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Abstract
Disclosed is a process for growing the microflora Thraustochytrium, Schizochytrium, and mixtures thereof, which includes the growing of the microflora in fermentation medium containing non-chloride containing sodium salts, in particular sodium sulfate. In a preferred embodiment of the present invention, the process produces microflora having a cell aggregate size useful for the production of food products for use in aquaculture. Further disclosed is a food product which includes Thraustochytrium, Schizochytrium, and mixtures thereof, and a component selected from flaxseed, rapeseed, soybean and avocado meal. Such a food product includes a balance of long chain and short chain omega-3 highly unsaturated fatty acids.
119 Citations
20 Claims
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1. A process for culturing a microorganism of the order Thraustochytriales for the production of lipids, the process comprising:
culturing the microorganism of the order Thraustochytriales in a culture medium that comprises a chloride concentration of less than about 500 mg/L and a non-chloride source of sodium selected from the group consisting of soda ash, sodium carbonate, sodium bicarbonate, sodium sulfate and mixtures thereof, and wherein the microorganism after culturing has a sterol content of at least about 0.1% ash-free dry weight (afdw) and a cholesterol content of at least about 15% of the total sterol content. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
Specification