Defined cell culturing surfaces and methods of use
First Claim
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1. A method of preparing a cell culturing substrate comprising:
- providing a cell culture surface;
plasma polymerizing a film onto said surface to form a film-coated surface, wherein said plasma polymerizing utilizes one or more monomers; and
introducing a coating solution to said film-coated surface to form a cell culture substrate, said coating solution comprising one or more extracellular matrix proteins and an aqueous solution, wherein the total extracellular matrix protein concentration in the coating solution is about 1 ng/mL to about 1 mg/mL.
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Abstract
In one aspect, there is provided a cell culturing substrate including: a cell culture surface having a film attached thereto, wherein the film includes one or more plasma polymerized monomers; and a coating on the film-coated surface, the coating deposited from a coating solution comprising one or more extracellular matrix proteins and an aqueous solvent, where the total extracellular matrix protein concentration in the coating solution is about 1 ng/mL to about 1 mg/mL.
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Citations
15 Claims
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1. A method of preparing a cell culturing substrate comprising:
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providing a cell culture surface; plasma polymerizing a film onto said surface to form a film-coated surface, wherein said plasma polymerizing utilizes one or more monomers; and introducing a coating solution to said film-coated surface to form a cell culture substrate, said coating solution comprising one or more extracellular matrix proteins and an aqueous solution, wherein the total extracellular matrix protein concentration in the coating solution is about 1 ng/mL to about 1 mg/mL. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification