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Defined cell culturing surfaces and methods of use

  • US 8,288,513 B2
  • Filed: 07/24/2009
  • Issued: 10/16/2012
  • Est. Priority Date: 07/25/2008
  • Status: Active Grant
First Claim
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1. A method of preparing a cell culturing substrate comprising:

  • providing a cell culture surface;

    plasma polymerizing a film onto said surface to form a film-coated surface, wherein said plasma polymerizing utilizes one or more monomers; and

    introducing a coating solution to said film-coated surface to form a cell culture substrate, said coating solution comprising one or more extracellular matrix proteins and an aqueous solution, wherein the total extracellular matrix protein concentration in the coating solution is about 1 ng/mL to about 1 mg/mL.

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