Functional molecule, functional molecule synthesizing amidite and target substance analysis method
First Claim
1. A functional molecule comprising:
- a modified nucleotide unit having a substituent removably introduced to a base thereof,wherein the substituent is removable by at least one method selected from the group consisting of a method involving an ammonia treatment, a method involving an alkali treatment using NaOH, a method involving a treatment using hydrofluoric acid and fluoride, a method involving a treatment using hydrazine, and a method involving irradiation of light; and
is not removable under a condition where a protection group for protecting the substituent used for synthesizing the functional molecule is removed by a method using a bulky base in an aprotic solvent, andwherein the functional molecule is convertible to be a molecule having a structure similar to that of naturally occurring nucleic acid by removing the substituent from the functional molecule.
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Accused Products
Abstract
To provide a functional molecule including a modified nucleotide unit having a substituent introduced to a base thereof, wherein the substituent is removably introduced to the base; a functional molecule synthesizing amidite that has a substituent removably introduced to its base and that is used for the manufacture of the functional molecule; and a target substance analysis method including: preparing a random pool of functional molecules using a functional molecule synthesizing amidite; screening a functional molecule having affinity for a target substance from the random pool; amplifying the functional molecules having affinity for the target substance, wherein the method further comprises, prior to the amplification step, removing a substituent from the functional molecule having affinity for the target substance.
3 Citations
7 Claims
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1. A functional molecule comprising:
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a modified nucleotide unit having a substituent removably introduced to a base thereof, wherein the substituent is removable by at least one method selected from the group consisting of a method involving an ammonia treatment, a method involving an alkali treatment using NaOH, a method involving a treatment using hydrofluoric acid and fluoride, a method involving a treatment using hydrazine, and a method involving irradiation of light; and
is not removable under a condition where a protection group for protecting the substituent used for synthesizing the functional molecule is removed by a method using a bulky base in an aprotic solvent, andwherein the functional molecule is convertible to be a molecule having a structure similar to that of naturally occurring nucleic acid by removing the substituent from the functional molecule. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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Specification