Fast results hybrid capture assay and system
First Claim
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1. A composition comprising:
- (a) a biological sample suspended in a collection medium, wherein said collection medium comprises about 0.5% to about 2.0% NP-40, about 0.10% to about 0.40% sodium deoxycholate, and about 10 mM to about 50 mM EDTA;
(b) a denaturation reagent;
(c) at least one polynucleotide probe capable of binding to a target nucleic acid molecule;
(d) a support coated with a first antibody; and
(e) a second antibody labeled with a detectable marker.
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Abstract
The present invention comprises a method that provides fast and reliable results for detecting the presence of a target nucleic acid molecule in a sample.
155 Citations
22 Claims
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1. A composition comprising:
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(a) a biological sample suspended in a collection medium, wherein said collection medium comprises about 0.5% to about 2.0% NP-40, about 0.10% to about 0.40% sodium deoxycholate, and about 10 mM to about 50 mM EDTA; (b) a denaturation reagent; (c) at least one polynucleotide probe capable of binding to a target nucleic acid molecule; (d) a support coated with a first antibody; and (e) a second antibody labeled with a detectable marker. - View Dependent Claims (2, 3)
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4. A composition comprising:
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(a) a biological sample suspended in a collection medium comprising about 0.5% to about 2.0% NP-40, about 0.10% to about 0.40% sodium deoxycholate, and about 10 mM to about 50 mM EDTA, and (b) a polynucleotide probe. - View Dependent Claims (5, 6, 7, 8, 9, 10)
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11. A kit for the detection of a target nucleic acid molecule in a sample, comprising
a) a collection medium comprising about 0.5% to about 2.0% NP-40, about 0.10% to about 0.40% sodium deoxycholate, and about 10 mM to about 50 mM EDTA; -
b) a denaturation reagent; c) a support coated with a first anti-hybrid antibody; d) a detection reagent comprising a second anti-poly hybrid antibody, wherein the second antibody is detectably labeled; e) a detergent-based wash buffer; and f) a second detection reagent comprising a substrate for the label on the second antibody; and (g) a polynucleotide probe. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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22. A kit for the detection of a target nucleic acid molecule in a sample, comprising
a) a collection medium comprising a detergent; -
b) a denaturation reagent; c) a support coated with a first anti-hybrid antibody; d) a detection reagent comprising a second anti-poly hybrid antibody, wherein the second antibody is detectably labeled; e) a detergent-based wash buffer comprising about 0.5% to about 2.0% NP-40, about 0.10% to about 0.40% sodium deoxycholate, about 25 mM to about 75 mM Tris-HCl, about 10 mM to about 50 mM EDTA, about 50 mM to about 200 mM NaCl, and about 0.01% to about 0.10% sodium azide; and f) a second detection reagent comprising a substrate for the label on the second antibody.
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Specification