Detection of nucleic acids through amplification of surrogate nucleic acids
First Claim
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1. A composition comprising:
- a first target nucleic acid;
a nucleic acid comprising a first surrogate nucleic acid;
a first set of two or more surrogate capture probes, each of which surrogate capture probes is capable of hybridizing simultaneously to the first target nucleic acid and to the nucleic acid comprising the first surrogate nucleic acid, wherein the 5′
end of each of the surrogate capture probes in the first set hybridizes to the first target nucleic acid while the 3′
end hybridizes to the nucleic acid comprising the first surrogate nucleic acid, or wherein the 3′
end of each of the surrogate capture probes in the first set hybridizes to the first target nucleic acid while the 5′
end hybridizes to the nucleic acid comprising the first surrogate nucleic acid;
a solid support;
a first set of m target capture probes, where m is at least one, which target capture probes are capable of hybridizing to the first target nucleic acid; and
one or more primers for amplifying the first surrogate nucleic acid.
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Abstract
Methods for detecting and optionally quantitating one or more target nucleic acids are provided, in which a surrogate nucleic acid is captured to each target nucleic acid, amplified, and detected. Compositions, kit, and systems related to the methods are also described.
48 Citations
16 Claims
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1. A composition comprising:
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a first target nucleic acid; a nucleic acid comprising a first surrogate nucleic acid; a first set of two or more surrogate capture probes, each of which surrogate capture probes is capable of hybridizing simultaneously to the first target nucleic acid and to the nucleic acid comprising the first surrogate nucleic acid, wherein the 5′
end of each of the surrogate capture probes in the first set hybridizes to the first target nucleic acid while the 3′
end hybridizes to the nucleic acid comprising the first surrogate nucleic acid, or wherein the 3′
end of each of the surrogate capture probes in the first set hybridizes to the first target nucleic acid while the 5′
end hybridizes to the nucleic acid comprising the first surrogate nucleic acid;a solid support; a first set of m target capture probes, where m is at least one, which target capture probes are capable of hybridizing to the first target nucleic acid; and one or more primers for amplifying the first surrogate nucleic acid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 16)
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14. A kit comprising:
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a nucleic acid comprising a first surrogate nucleic acid; a first set of two or more surrogate capture probes, each of which surrogate capture probes is capable of hybridizing simultaneously to a first target nucleic acid and to the nucleic acid comprising the first surrogate nucleic acid, wherein the 5′
end of each of the surrogate capture probes in the first set hybridizes to the first target nucleic acid while the 3′
end hybridizes to the nucleic acid comprising the first surrogate nucleic acid, or wherein the 3′
end of each of the surrogate capture probes in the first set hybridizes to the first target nucleic acid while the 5′
end hybridizes to the nucleic acid comprising the first surrogate nucleic acid;a solid support comprising a first support capture probe bound to the solid support; a first set of m target capture probes, where m is at least one, which target capture probes are capable of hybridizing simultaneously to the first target nucleic acid and to the first support capture probe; and one or more primers for amplifying the first surrogate nucleic acid; packaged in one or more containers. - View Dependent Claims (15)
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Specification