Process for production of porous body and uses thereof

• US 8,293,046 B2
• Filed: 03/24/2008
• Issued: 10/23/2012
• Est. Priority Date: 03/30/2007
• Status: Expired due to Fees

• Current Assignee: JMS Company Limited
• Original Assignee: JMS Company Limited
• Inventors: Sajiki, Toshinobu, Hirai, Satoshi
• Primary Examiner(s): Goff, John
• Application Number:
  US12/518,386
• Publication Number:
  US 20090319016A1
• Time in Patent Office: 1,674 Days
• Field of Search: 156/80, 156/242, 156/281, 156/308.2, 156/309.9, 623/23.72, 623/23.76, 424/423, 424/426
• US Class Current: 156/80
• CPC Class Codes: A61L 27/56 Porous materials, e.g. foams or sponges
• CPC Class Codes: C08J 2201/052 Inducing phase separation by thermal treatment, e.g. cooling a solution
• CPC Class Codes: C08J 2367/04 Polyesters derived from hydroxy carboxylic acids, e.g. lactones C08J2367/06 takes precedence
• CPC Class Codes: C08J 2467/00 Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain of polyester-amides C08J2477/12; of polyester-imides C08J2479/08; Derivatives of such polymers
• CPC Class Codes: C08J 9/0061 characterized by the use of several polymeric components
• CPC Class Codes: C08J 9/28 by elimination of a liquid phase from a macromolecular composition or article, e.g. drying of coagulum

Abstract

A process for producing a porous body with different physical properties in desired regions is provided by pore forming treatment, not by bonding two or more materials made porous beforehand, with, for example, an adhesive. Raw materials are prepared, each of which contains a polymer and a raw material preparation solvent. At least two types of the raw materials having different compositions are prepared. Thereafter, the respective raw materials are frozen into desired shapes to form frozen bodies thereof. The frozen bodies thus formed are brought into contact with each other to form an assembly thereof, the assembly is exposed to a condition under which the frozen bodies begin to melt, and the assembly is then freeze-dried. Thus, a porous body having regions different in physical properties can be obtained. Such a porous body can be used as, for example, an adhesion inhibitory material or a scaffold for a cell culture.

Claims

1. A process for producing a porous body with different physical properties in desired regions,

wherein the process comprises the following (A) to (D):

(A) providing at least two raw materials having different compositions, each of which contains a polymer and a solvent, wherein at least one of the raw materials is a gelled product that is obtained by the following (A1) to (A3);

(A1) mixing the polymer and the solvent to prepare a mixture,

(A2) allowing the mixture to stand to separate the mixture into a liquid phase and a gel phase, and

(A3) recovering the gel phase as the gelled product,

(B) freezing the respective raw materials into desired shapes to form frozen bodies of the respective raw materials,

(C) forming an assembly of the frozen bodies, where at least one of the following (C1) and (C2) is included,

(C1) forming the assembly of the frozen bodies and then exposing the assembly to a condition under which the frozen bodies begin to melt, and

(C2) exposing the respective frozen bodies to a condition under which the frozen bodies begin to melt and then forming the assembly of the frozen bodies, and

(D) freeze-drying the assembly after (C) to form a porous body.

2. The process according to claim 1, wherein the porous body to be formed is a porous body with physical properties varying in its thickness direction, and in (C), the frozen bodies are laminated together to form a laminate of the frozen bodies.

3. The process according to claim 1, wherein the physical properties are at least one selected from the group consisting of an average pore size, porosity, strength, thickness, material permeability, and adhesiveness.

4. The process according to claim 1, wherein in (B), the respective raw materials are frozen under a temperature condition that is equal to or lower than eutectic points thereof.

5. The process according to claim 1, wherein in (B), the respective raw materials are frozen at lower than 0°

C.

6. The process according to claim 1, wherein in (C1), the assembly is exposed to a temperature condition that is at least the highest melting point among those of the respective frozen bodies that form the assembly.

7. The process according to claim 1, wherein the assembly in (C1) or the frozen bodies in (C2) are exposed to a temperature condition between 0°

C. and 25°

C.

8. The process according to claim 6, wherein in (C1) or (C2), a treatment time under the condition is at least 30 seconds.

9. The process according to claim 1, wherein the polymer is a lactide-caprolactone copolymer.

10. The process according to claim 9, wherein in the lactide-caprolactone copolymer, the molar ratio between lactide and caprolactone is in a range of 90:

10 to 10;

90.

11. The process according to claim 1, wherein the solvent is at least one of a solvent having solubility to the polymer and a mixed solvent containing a solvent having solubility to the polymer and a solvent having insolubility to the polymer.

12. The process according to claim 1, wherein at least one of the raw materials used in (A) is a solution containing the polymer dissolved in the solvent.

13. The process according to claim 12, wherein the amount of the polymer in the solution is 0.1 to 24 wt % based on the total weight of the solution.

14. The process according to claim 1, wherein at least one of the raw materials used in (A) is a solution containing a lactide-caprolactone copolymer dissolved in the solvent,

the solvent contains a solvent having solubility to the copolymer, and

the amount of the solvent having solubility to the copolymer is more than 84.48 wt % based on the total weight of the raw material.

15. The process according to claim 14, wherein the solvent is a mixed solvent containing a solvent having solubility to the copolymer and a solvent having insolubility to the copolymer, and

the amount of the solvent having insolubility is lower than 12 wt % based on the total weight of the raw material.

16. The process according to claim 1, wherein the amount of the polymer in the mixture obtained in (A1) is 0.1 to 24 wt % based on the total weight of the mixture obtained in (A1).

17. The process according to claim 1, wherein the polymer used in (A1) is a lactide-caprolactone copolymer,

the solvent is a mixed solvent containing a solvent having solubility to the copolymer and a solvent having insolubility to the copolymer, and

the amount of the solvent having insolubility is 12 to 40 wt % based on the total weight of the mixture obtained in (A1).

18. The process according to claim 11, wherein the solvent having solubility to the polymer is at least one selected from the group consisting of 1,4-dioxane, dimethyl carbonate, chloroform, and acetone.

19. The process according to claim 11, wherein the solvent having insolubility to the polymer is at least one solvent selected from the group consisting of water, ethanol, tertiary butyl alcohol, and hexane.