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Assessment of reaction kinetics compatibility between polymerase chain reactions

  • US 8,293,473 B2
  • Filed: 04/04/2006
  • Issued: 10/23/2012
  • Est. Priority Date: 04/04/2006
  • Status: Expired due to Fees
First Claim
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1. A method for the assessment of compatibility of an individual PCR with a defined reference of PCRs comprising the steps of:

  • a) amplifying by PCR a nucleic acid together with a signaling agent in an investigated sample;

    b) obtaining a reference set by amplifying by PCR a plurality of samples with a reference nucleic acid sequence together with a signaling agent;

    c) obtaining a time series of signals from every PCR by reading and storing at a plurality of time points at least one signal whose intensity is related to the quantity of the nucleic acid sequence formed in the PCR;

    d) from the time series of signals of every PCR, deriving a plurality of parameters characterizing change of the signal accompanying the reaction progression;

    e) simultaneously comparing by a single statistical test the parameters of the investigated PCR with the parameters of the PCRs of the reference set;

    f) based on the probability obtained from the statistical test, drawing an inference on the geometry of the reaction kinetics of the investigated PCR with the reference set, wherein a parallel geometry indicates reaction kinetics compatible between the investigated PCR and the reference set; and

    g) informing a user about one or more PCRs with non-parallel geometry if this is elicited in step f.

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