Three-dimensional ex vivo system

• US 8,293,531 B1
• Filed: 08/29/2008
• Issued: 10/23/2012
• Est. Priority Date: 08/31/2007
• Status: Active Grant

• Current Assignee: Clemson University
• Original Assignee: Clemson University
• Inventors: Burg, Karen J. L., Yang, Chih-Chao
• Primary Examiner(s): Saidha, Tekchand
• Assistant Examiner(s): Meah, Younus
• Application Number:
  US12/201,297
• Time in Patent Office: 1,516 Days
• Field of Search: None
• US Class Current: 435/398
• CPC Class Codes: C12N 2533/30 Synthetic polymers thermoreactive polymers, e.g. PNIPAm, C12N2539/10
• CPC Class Codes: C12N 2533/74 Alginate
• CPC Class Codes: C12N 5/0062 General methods for three-dimensional culture

Abstract

Disclosed are three-dimensional (3D) systems as may be utilized for ex vivo tissue or cell growth and development. A system generally includes a base material and at least one wicking fiber embedded therein through which a liquid can be spontaneously drawn by capillary action. Wicking fibers can define a plurality of colinear channels along the exterior surface of the axial length of the fiber. Wicking fibers can be present in disclosed systems as individual fibers or in bundles. Disclosed systems can be useful in various scientific studies, including, but not limited to, drug discovery, vaccine development, cell biology studies, and biomaterial development.

Claims

1. A three-dimensional ex vivo or in vitro system comprising:

a base material;

a bundle of fibers comprising a plurality of wicking fibers embedded within the base material, each wicking fiber defining a plurality of colinear channels on the exterior surface of the wicking fiber, the colinear channels extending along the length of the exterior surface of the fiber, the bundle of fibers comprising the plurality of wicking fibers aligned with one another; and

a plurality of ex vivo or in vitro cells, wherein

the system supports the growth and development of the ex vivo or in vitro cells.

2. The three-dimensional ex vivo or in vitro system according to claim 1, wherein each wicking fiber is a hollow fiber.

3. The three-dimensional ex vivo or in vitro system according to claim 1, wherein the base material is a hydrogel.

4. The three-dimensional ex vivo or in vitro system according to claim 1, wherein the base material is an alginate sponge.

5. The three-dimensional ex vivo or in vitro system according to claim 1, wherein the system is sterile.

6. The three-dimensional ex vivo or in vitro system according to claim 1, further comprising one or more noncellular biologically active materials.

7. The three-dimensional ex viva or in vitro system according to claim 1, wherein the system is a fiber bioreactor system.

8. The three-dimensional ex viva or in vitro system according to claim 1, wherein the system is a component of a dialysis system.

9. The three-dimensional ex vivo or in vitro system according to claim 1, wherein the plurality of cells comprise a variety of cell types.

10. The three-dimensional ex vivo or in vitro system according to claim 1, wherein the plurality of cells are a portion of a tissue explant.

11. The three-dimensional ex vivo or in vitro system according to claim 1, wherein the base material is a gel matrix or a viscous fluid matrix.

12. The three-dimensional ex vivo or in vitro system according to claim 1, wherein the plurality of cells are autologous, allogenic, or xenogenic cells.

13. The three-dimensional ex vivo or in vitro system according to claim 6, wherein a noncellular biologically active material is covalently linked or associated by affinity to at least one of the base material and the bundle of fibers.

14. The three-dimensional ex vivo or in vitro system according to claim 1, wherein the cells are seeded on or in the wicking fibers.

15. The three dimensional ex vivo or in vitro system according to claim 1, wherein the cells are incorporated into the base material.

16. The three-dimensional ex vivo or in vitro system according to claim 1, further comprising a well comprising a liquid at one end of the bundle of fibers.