Method and apparatus for tissue transfer

• US 8,293,532 B2
• Filed: 03/16/2010
• Issued: 10/23/2012
• Est. Priority Date: 03/26/2009
• Status: Active Grant

• Current Assignee: Corteva Agriscience LLC (Corteva, Inc.)
• Original Assignee: Dow Agrosciences LLC (Corteva, Inc.)
• Inventors: Moynahan, Tonya Lynne Strange
• Primary Examiner(s): PARA, ANNETTE H
• Application Number:
  US12/724,965
• Publication Number:
  US 20100248370A1
• Time in Patent Office: 952 Days
• Field of Search: None
• US Class Current: 435/430
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 3/0275 Interchangeable or disposable dispensing tips
• CPC Class Codes: B25J 15/0616 with vacuum
• CPC Class Codes: B25J 19/04 Viewing devices
• CPC Class Codes: B25J 9/1697 Vision controlled systems
• CPC Class Codes: C12M 33/04 by injection or suction, e.g. using pipettes, syringes, needles pipettes in general B01L3/02
• CPC Class Codes: C12M 41/48 Automatic or computerized control automatic analysis G01N35/00
• CPC Class Codes: G01N 1/28 Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Qmounting specimens on microscopic slides G02B21/34; means for supporting the objects or the materials to be analysed in electron microscopes H01J37/20 ; laboratory gas handling apparatus B01L5/00
• CPC Class Codes: Y10S 901/09 Closed loop, sensor feedback controls arm movement
• CPC Class Codes: Y10S 901/40 Vacuum or mangetic
• CPC Class Codes: Y10S 901/47 Optical

Abstract

A handheld tool is disclosed which may be used to transfer a plurality of plant tissue explants from a first container to a second container. The handheld tool may include a disposable tip member which couples the plurality of plant tissue explants through use of negative pressure. An automated system which transfers a plurality of plant tissue explants from a first container to a second container is also disclosed. The automated system may include a first presentment system which moves the first container to a region, a second presentment system which moves the second container to the region, and a robot system that transfers the plurality of plant tissue explants from the first container to the second container.

Claims

1. A method of transferring plant tissue explants from a first container having a first nutrient medium substrate adapted to provide nutrients to the plant tissue explants to a second container having a second nutrient medium substrate adapted to provide nutrients to the plant tissue explants, the method comprising the steps of:

(a) coupling a first plant tissue explant to a handheld tool, the handheld tool coupling the first plant tissue explant through a negative pressure present in an interior of the handheld tool;

(b) generally separating the first plant tissue explant from the first nutrient medium substrate adapted to provide nutrients to the first plant tissue explant and from the first container;

(c) locating the first plant tissue explant relative to the second nutrient medium substrate adapted to provide nutrients to the first plant tissue explant of the second container; and

(d) uncoupling the first plant tissue explant from the handheld tool.

2. The method of claim 1, wherein the handheld tool includes a fluid conduit having a first end and a second end, the second end of the fluid conduit being in fluid communication with the first end of the fluid conduit and the fluid conduit having at least one vent passage positioned between the first end of the fluid conduit and the second end of the fluid conduit and being in fluid communication with the first end of the fluid conduit and the second end of the fluid conduit and to the surrounding environment, the vent passage being blocked from fluid communication with the surrounding environment during steps (a) through (c) with a stop device and the vent passage being in fluid communication with the surrounding environment during step (d).

3. The method of claim 2, wherein stop device is one of an operator'"'"'s finger and a device supported by the operator'"'"'s finger.

4. The method of claim 2, wherein the fluid conduit is an assembly and includes at least a first portion and a second portion, both the first portion and the second portion having a respective interior in fluid communication with the first end of the handheld tool and the second end of the handheld tool when the first portion and the second portion are assembled, the method further comprising the steps of:

(e) providing a plurality of first portions of the handheld tool;

(f) assembling a first one of the plurality of first portions of the handheld tool to the second portion of the handheld tool;

(g) performing steps (a) through (d) for all of the plant tissue explants in the first container selected for transfer to the second container;

(h) separating the first one of the plurality of first portions of the handheld tool from the second portion of the handheld tool;

(i) obtaining a third container including another plurality of plant tissue explants for transfer to a fourth container;

(j) assembling a second one of the plurality of first portions of the handheld tool to the second portion of the handheld tool; and

(k) performing steps (a) through (d) for all of the plant tissue explants in the third container selected for transfer to the fourth container.

5. The method of claim 4, wherein the plurality of first portions are a plurality of pipette tips.

6. The method of claim 5, wherein the plurality of pipette tips are stored generally vertical in a pipette tip box with a tip portion lower than a coupling portion, the step of assembling a first one of the plurality of first portions of the handheld tool to the second portion of the handheld tool includes the step inserting a portion of the second portion of the handheld tool into the coupling portion of a first pipette tip of the plurality of pipette tips while the first pipette tip is stored in the pipette tip box.

7. The method of claim 1, wherein steps (a) through (d) are performed in an aseptic environment.

8. The method of claim 7, wherein steps (a) through (d) are performed in a laminar flow hood.

9. The method of claim 1, wherein the step of uncoupling the first plant tissue explant from the handheld tool includes the step of reducing the negative pressure in the handheld tool by opening a vent passage of the handheld tool.

10. The method of claim 1, further comprising the step of preventing the first plant tissue explant from entering into an interior of the handheld tool.

11. The method of claim 10, wherein the first plant tissue explant is coupled to a first end of the handheld tool, the first end of the handheld tool including an opening sized to prevent the first plant tissue explant from entering into the interior of the handheld tool.

12. The method of claim 1, wherein during step (c) the first plant tissue explant is held stationary relative to the handheld tool.

13. The method of claim 2, further comprising the step of filtering the fluid as it travels between the first end of the fluid conduit and the second end of the fluid conduit.