Thyroid analyte measurement

• US 8,293,541 B2
• Filed: 05/13/2010
• Issued: 10/23/2012
• Est. Priority Date: 11/12/2008
• Status: Active Grant

• Current Assignee: Hemopet
• Original Assignee: Hemopet
• Inventors: Dodds, W. Jean, Ongchango, Ferdie S.
• Primary Examiner(s): Counts, Gary W
• Application Number:
  US12/779,196
• Publication Number:
  US 20110111521A1
• Time in Patent Office: 894 Days
• Field of Search: None
• US Class Current: 436/501
• CPC Class Codes: G01N 33/78 Thyroid gland hormones , e.g. T3, T4, TBH, TBG or their receptors

Abstract

A non-radioisotopic method measures Free T3 and/or Free T4 in a sample of a non-human species, which can be a canine.

Claims

1. A method of measuring thyroid analytes comprising:

a. obtaining a patient sample from a canine species;

b. incubating the sample with a label detectant;

c. measuring Free T4 in the sample of the canine species using the label as the detectant, and

d. measuring T4 in the sample; and

e. applying a r-squared value of the ratio of T4 relative to Free T4 between about 0.75 and about 0.99 as a correlation to establish a diagnostic specificity for canine thyroid disease.

8. A method as claimed in claim 1 included in a panel for measuring thyroid activity of the sample, and the panel consisting additionally of non-radio isotopic measurement of T3, and Free T3 in the sample of biological fluid from the canine.

9. The method as claimed in claim 8 consisting of adding the sample to a luminescence reaction assay for measuring T3, Free T3, T4 and Free T4 in the canine species, and measuring the amount of luminescence from a reaction of the sample with a reactant in the assay, and thereby determining the amount of T3, Free T3, T4 and Free T4 in the canine species, including employing a luminescent detector and microparticles as a solid phase for measuring T3, Free T3, T4, Free T4 in the canine species.

10. The method of claim 8 applying a luminescent detector and microparticles as a solid phase.

11. The method of claim 8 including applying chemiluminescence.

12. The method of claim 8 including measuring Free T4 by applying a luminescent detector and microparticles as a solid phase, and effecting the detection with a sensitivity of about 1 femtogram per mL.

13. The method of claim 8 including measuring Free T4 by chemiluminescence, and having an incubation period for less than 90 minutes.

19. The method of claim 1 wherein the label detectant is a non-radioisotopic label.

2. A method of measuring thyroid analyte comprising:

a. obtaining a patient sample from a canine species;

b. incubating the sample with a label detectant;

c. measuring T4 in the sample;

d. measuring Free T4 in the sample of the canine species using the label as the detectant, and effecting the measurement with a sensitivity of about 1 femtogram per mL;

e. employing direct chemiluminescence, and including a bioluminescent detector and microparticles as a solid phase for immobilizing FreeT4; and

f. applying a r-squared value of the ratio of T4 relative to Free T4 between about 0.75 and about 0.99 as a correlation to establish a diagnostic specificity for canine thyroid disease.

3. The method as claimed in claim 2 including employing paramagnetic particles as the solid phase.

5. The method as claimed in claim 2 including having an incubation period between 15 and 90 minutes.

6. The method as claimed in claim 3 including having an incubation period between 15 and 90 minutes.

20. The method of claim 2 wherein the label detectant is a non-radioisotopic label.

4. A method of measuring thyroid analyte comprising:

a. obtaining a patient sample from a canine species;

b. incubating the sample with a label detectant;

c. measuring T4 in the sample;

d. measuring Free T4 in the sample of the canine species using the label as the detectant; and

employing direct chemiluminescence, and including a bioluminescent detector and microparticles as a solid phase for immobilizing FreeT4 including having an incubation period for less than 90 minutes, and applying a r-squared value of the ratio of T4 relative to Free T4 between about 0.75 and about 0.99 as a correlation to establish a diagnostic specificity for canine thyroid disease.

7. The method as claimed in claim 4 including having an incubation period for less than 90 minutes.

21. The method of claim 4 wherein the label detectant is a non-radioisotopic label.

14. A method of diagnosing thyroid disease in a canine wherein the method includes a method of measuring thyroid analytes comprising:

a) obtaining a patient sample from a canine species;

b) incubating the sample with a label detectant;

c) measuring Free T4 in the sample of the canine species using the label as the detectant;

d) measuring T4 in the sample; and

e) applying a r-squared value of the ratio of T4 relative to Free T4 between about 0.75 and about 0.99, as a correlation to establish a diagnostic specificity for canine thyroid disease such that thyroid disease can be diagnosed.

15. The method of claim 14 including measuring Free T4 in the sample with a sensitivity of about 1 femtogram per mL, employing direct chemiluminescence, and including a bioluminescent detector and microparticles as a solid phase for immobilizing FreeT4.

16. The method of claim 14 including employing paramagnetic particles as the solid phase.

17. The method of claim 14 including having an incubation period for less than 90 minutes.

18. The method of claim 14 including having an incubation period between 15 and 90 minutes.

22. The method of claim 14 wherein the label detectant is a non-radioisotopic label.