Transition metal complexes with (pyridyl)imidazole ligands

• US 8,298,388 B2
• Filed: 09/10/2009
• Issued: 10/30/2012
• Est. Priority Date: 05/11/2001
• Status: Expired due to Term

• Current Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories Incorporated)
• Original Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories Incorporated)
• Inventors: Mao, Fei, Heller, Adam
• Primary Examiner(s): Olsen, Kaj K
• Application Number:
  US12/557,377
• Publication Number:
  US 20100038242A1
• Time in Patent Office: 1,146 Days
• Field of Search: 204/403.14, 204/403.04, 204/403.1, 526/161, 546/2, 548/101
• US Class Current: 204/403.04
• CPC Class Codes: C07F 15/0026 without a metal-carbon linkage

Abstract

Novel transition metal complexes of iron, cobalt, ruthenium, osmium, and vanadium are described. The transition metal complexes can be used as redox mediators in enzyme-based electrochemical sensors. The transition metal complexes include substituted or unsubstituted (pyridyl)imidazole ligands. Transition metal complexes attached to polymeric backbones are also described.

Claims

1. A test strip comprising:

a working electrode;

a counter electrode;

wherein the working electrode comprises an analyte-responsive enzyme and a mediator complex, the mediator complex having the formula;

2. The test strip of claim 1, wherein

M is osmium;

L₂ is a halide; and

L and L′

are independently;

3. The test strip of claim 2, wherein L₁ has the formula:

4. The test strip of claim 3, wherein R₁₁ and R₁₅ are independently —

H;

R₁₂ and R₁₄ are the same and —

H; and

R₁₃ is methylamino.

5. The test strip of claim 2, wherein L₂ is —

F, —

Cl, or —

Br.

6. The test strip of claim 2, wherein L₂ is —

Cl.

7. The test strip of claim 2, wherein R′

₁ is a C1-C2 alkyl.

8. The test strip of claim 2, wherein R′

₁ is a C1 alkyl.

9. The test strip of claim 2, wherein each of R_a and R_c is —

H.

10. The test strip of claim 2, wherein each of R_a, R_b, and R_c is —

H.

11. The test strip of claim 2, wherein each of R_a, R_b, R_c, and R_d is —

H.

12. The test strip of claim 2, wherein X is a halide.

13. The test strip of claim 2, wherein X is chloride.

14. The test strip of claim 1, wherein the analyte-responsive enzyme is glucose dehydrogenase (GDH).

15. The test strip of claim 1, wherein the analyte-responsive enzyme further comprises an enzyme cofactor.

16. A test strip comprising:

a working electrode;

a counter electrode;

wherein the working electrode comprises an analyte-responsive enzyme and a mediator complex, the mediator complex having the formula;

17. The test strip of claim 16, wherein the analyte-responsive enzyme is glucose dehydrogenase (GDH).

18. The test strip of claim 16, wherein the analyte-responsive enzyme further comprises an enzyme cofactor.

19. A test strip comprising:

a working electrode;

a counter electrode;

wherein the working electrode comprises an analyte-responsive enzyme and a mediator complex, the mediator complex having the formula;

20. The test strip of claim 19, wherein the analyte-responsive enzyme is glucose dehydrogenase (GDH).

21. The test strip of claim 19, wherein the analyte-responsive enzyme further comprises an enzyme cofactor.