Expression systems using mammalian beta-actin promoter
First Claim
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1. A DNA construct comprising a mouse β
- -actin promoter operably linked to a human Cytomegalovirus (CMV) enhancer.
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Abstract
Promoter activities were examined by comparing combinations of promoters and enhancers derived from various genes. A hybrid promoter comprising a combination of a CMV enhancer and a mammalian β-actin promoter, or the post-transcriptional regulatory region of the genomic sequence Woodchuck Hepatitis Virus (WPRE) and a mammalian β-actin promoter was found to be stronger than existing promoters. Furthermore, the activities of the β-actin promoters could be enhanced by coexpressing the oncogene product Ras, which is a transactivator.
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34 Claims
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1. A DNA construct comprising a mouse β
- -actin promoter operably linked to a human Cytomegalovirus (CMV) enhancer.
- View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 25, 26, 27, 29, 30, 31, 33, 34)
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2. A DNA construct comprising a mouse β
- -actin promoter operably linked to a CMV enhancer, wherein the CMV enhancer comprises the nucleotide sequence shown in SEQ ID NO;
4 and the mouse β
-actin promoter comprises the nucleotide sequence shown in SEQ ID NO;
2. - View Dependent Claims (24, 28, 32)
- -actin promoter operably linked to a CMV enhancer, wherein the CMV enhancer comprises the nucleotide sequence shown in SEQ ID NO;
Specification