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System and method for correcting primer extension errors in nucleic acid sequence data

  • US 8,301,394 B2
  • Filed: 02/15/2007
  • Issued: 10/30/2012
  • Est. Priority Date: 02/16/2006
  • Status: Active Grant
First Claim
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1. A method for correcting an error associated with phasic synchrony of sequence data generated from a population of substantially identical copies of a template nucleic acid molecule, comprising:

  • (a) detecting a signal generated in response to an incorporation of one or more nucleotides in a sequencing reaction, wherein the signal is generated from the population of substantially identical copies of the template nucleic acid molecule;

    (b) generating a signal value for the signal;

    (c) repeating steps (a)-(b) for a plurality of sequence positions of the template nucleic acid molecule;

    (d) correcting a plurality of the signal values for a phasic synchrony error using a first parameter representative of an incomplete extension component of the phasic synchrony error that comprises a measure of one or more of the substantially identical copies of the template nucleic acid molecules behind an extension phase of the population and a second parameter representative of a carry forward component of the phasic synchrony error that comprises a measure of one or more of the substantially identical copies of the template nucleic acid molecules ahead of the extension phase of the population.

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