Nucleic acid sequencing methods and systems
First Claim
Patent Images
1. A sequencing system, the system comprising:
- (a) a reaction chamber, wherein the reaction chamber contains a sequencing reaction mixture comprising;
(i) a template nucleic acid;
(ii) a complementary nucleic acid that is at least partially complementary to the template nucleic acid, wherein the complementary nucleic acid is hybridized to the template nucleic acid;
(iii) an exonuclease that comprises template dependent nucleobase binding activity, wherein the exonuclease is substantially free of nucleotide polymerization activity, and(iv) at least one labeled nucleobase;
(b) a detector configured to detect a signal from the reaction chamber, which signal results from a transient template-dependent binding of a labeled nucleobase by the exonuclease in the reaction chamber; and
,(c) a sequence assembly module that assembles template nucleic acid sequence information based upon detection of the signal.
0 Assignments
0 Petitions
Accused Products
Abstract
Sequencing methods that use an exonuclease that comprises template dependent nucleobase binding activity are provided. Related compositions and sequencing systems are also provided.
-
Citations
15 Claims
-
1. A sequencing system, the system comprising:
-
(a) a reaction chamber, wherein the reaction chamber contains a sequencing reaction mixture comprising; (i) a template nucleic acid; (ii) a complementary nucleic acid that is at least partially complementary to the template nucleic acid, wherein the complementary nucleic acid is hybridized to the template nucleic acid; (iii) an exonuclease that comprises template dependent nucleobase binding activity, wherein the exonuclease is substantially free of nucleotide polymerization activity, and (iv) at least one labeled nucleobase; (b) a detector configured to detect a signal from the reaction chamber, which signal results from a transient template-dependent binding of a labeled nucleobase by the exonuclease in the reaction chamber; and
,(c) a sequence assembly module that assembles template nucleic acid sequence information based upon detection of the signal. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
-
Specification