Methods and compositions for degradation of lignocellulosic material

• US 8,304,212 B2
• Filed: 07/10/2007
• Issued: 11/06/2012
• Est. Priority Date: 07/10/2006
• Status: Active Grant

• Current Assignee: Battelle Memorial Institute, Dyadic International (Usa), Inc., Iowa Corn Promotion Board
• Original Assignee: Battelle Memorial Institute, Dyadic International (Usa), Inc., Iowa Corn Promotion Board
• Inventors: Burlingame, Richard, Bradford, Marion, Sinitsyn, Arkady Panteleimonovich, Baez-Vasquez, Marco A., Magnuson, Jon K.
• Primary Examiner(s): FRONDA, CHRISTIAN L
• Application Number:
  US11/775,777
• Publication Number:
  US 20080076159A1
• Time in Patent Office: 1,946 Days
• Field of Search: None
• US Class Current: 435/72
• CPC Class Codes: C12N 9/2428 Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
• CPC Class Codes: C12N 9/2445 Beta-glucosidase (3.2.1.21)
• CPC Class Codes: C12P 19/02 Monosaccharides 2-ketogulonic acid C12P7/60
• CPC Class Codes: C12P 19/20 produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
• CPC Class Codes: C12Y 302/01003 Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
• CPC Class Codes: C12Y 302/01021 Beta-glucosidase (3.2.1.21)
• CPC Class Codes: C12Y 302/01037 Xylan 1,4-beta-xylosidase (3.2.1.37)
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

Methods to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material are provided, as well as novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass.

Claims

1. A multi-enzyme product comprising isolated enzymes from a Trichoderma and exhibiting:

a glucoamylase activity of at least about 0.001 U per milligram of dry matter, a β

-xylosidase activity of at least about 280 nmol/min/mg of dry matter, a β

-glucosidase activity of at least about 454 nmol/min/mg of dry matter, and an α

-arabinofuranosidase activity of at least about 100 nmol/min/mg of dry matter, wherein the multi-enzyme product increases the yield of fermentable sugars from Distiller'"'"'s Dried Grains (DDG) over compositions not including the claimed amount of glucoamylase.

2. The multi-enzyme product of claim 1, wherein the product is a crude fermentation product.

3. The multi-enzyme product of claim 1, wherein the product has been subjected to a purification step.

4. The multi-enzyme product of claim 1, wherein the Trichoderma is Trichoderma reesei.

5. The multi-enzyme product of claim 1, wherein the product is capable of converting lignocellulosic material to sugars.

6. The multi-enzyme product of claim 1, wherein the fermentable sugars comprise glucose.

7. The multi-enzyme product of claim 1, wherein the DDG is derived from corn.