Systems and methods for monitoring the amplification and dissociation behavior of DNA molecules
First Claim
Patent Images
1. A DNA analysis system, comprising:
- a chip comprising a microchannel for receiving a sample of solution comprising nucleic acid and for providing a path for the sample to traverse;
an image sensor having a pixel array, wherein at least a portion of the microchannel is within a field of view of the pixel array; and
an image sensor controller configured to define a first window of the pixel array and to define a second window of the pixel array for (a) reading the first window of the pixel array at a time when the sample is within a field of view of the first window, thereby producing first image data, and for (b) reading the second window of the pixel array at a time when the sample is within a field of view of the second window, whereinthe center of the second window is spaced apart from the center of the first window.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention relates to systems and methods for monitoring the amplification of DNA molecules and the dissociation behavior of the DNA molecules.
-
Citations
18 Claims
-
1. A DNA analysis system, comprising:
-
a chip comprising a microchannel for receiving a sample of solution comprising nucleic acid and for providing a path for the sample to traverse; an image sensor having a pixel array, wherein at least a portion of the microchannel is within a field of view of the pixel array; and an image sensor controller configured to define a first window of the pixel array and to define a second window of the pixel array for (a) reading the first window of the pixel array at a time when the sample is within a field of view of the first window, thereby producing first image data, and for (b) reading the second window of the pixel array at a time when the sample is within a field of view of the second window, wherein the center of the second window is spaced apart from the center of the first window. - View Dependent Claims (2, 3, 4, 5)
-
-
6. A DNA analysis method comprising:
-
introducing a sample of a solution comprising nucleic acid into a microchannel; causing the sample to move though the microchannel; defining a first window of a pixel array of an image sensor; defining a second window of the pixel array, wherein the center of the second window is spaced apart from the center of the first window; while the sample is moving through the microchannel, performing the steps of; (a) while the sample is within a field of view of the first window, windowing the image sensor so that image data from the first window is output to a data buffer, wherein said image data comprises data from which the intensity of emissions from the sample can be determined; and (b) after performing step (a), while the sample is within a field of view of the second window, windowing the image sensor so that image data from the second window is output to a data buffer, wherein said image data comprises data from which the intensity of emissions from the sample can be determined. - View Dependent Claims (7, 8, 9, 10)
-
-
11. A DNA analysis method comprising:
-
introducing a first sample of a solution comprising nucleic acid into a first microchannel; introducing a second sample of a solution comprising nucleic acid into a second microchannel; causing the first sample to move though the first microchannel; causing the second sample to move though the second microchannel; defining a first window of a pixel array of an image sensor, wherein at least a portion of the first microchannel is within the field of view of the first window; defining a second window of the pixel array, wherein at least a portion of the second microchannel is within the field of view of the second window and wherein the center of the second window is spaced apart from the center of the first window; while the samples are moving through the respective microchannels, performing the steps of; (a) while at least one of the samples is within a field of view of the first window, windowing the image sensor so that image data from the first window is output to a data buffer, wherein said image data comprises data from which the intensity of emissions from the first sample can be determined; and (b) after performing step (a), while at least one of the samples is within a field of view of the second window, windowing the image sensor so that image data from the second window is output to a data buffer, wherein said image data comprises data from which the intensity of emissions from the second sample can be determined. - View Dependent Claims (12, 13)
-
-
14. A DNA analysis device, comprising:
-
an image sensor having a pixel array, wherein at least a portion of a microchannel providing a path for a sample of solution comprising nucleic acid to traverse is within a field of view of the pixel array; and an image sensor controller configured to define a first window of the pixel array and to define a second window of the pixel array for (a) reading the first window of the pixel array at a time when the sample is within a field of view of the first window, thereby producing first image data, and for (b) reading the second window of the pixel array at a time when the sample is within a field of view of the second window, wherein the center of the second window is spaced apart from the center of the first window. - View Dependent Claims (15, 16, 17, 18)
-
Specification