System and method for treating wastewater via phototactic heterotrophic microorganism growth

• US 8,308,944 B2
• Filed: 03/19/2010
• Issued: 11/13/2012
• Est. Priority Date: 03/20/2009
• Status: Expired due to Fees

• Current Assignee: Algal Scientific Corporation
• Original Assignee: Algal Scientific Corporation
• Inventors: Horst, Geoff
• Primary Examiner(s): Prince, Fred
• Application Number:
  US12/727,738
• Publication Number:
  US 20100237009A1
• Time in Patent Office: 970 Days
• Field of Search: 210/602, 210/610, 210/611, 210/620, 210/630, 210/748.1, 210/205, 210/206, 210/221.2, 210/252, 210/259
• US Class Current: 210/602
• CPC Class Codes: C02F 1/30 by irradiation
• CPC Class Codes: C02F 1/66 by neutralisation; pH adjustment for degassing C02F1/20; using ion-exchange C02F1/42; for flocculation or precipitation of suspended impurities C02F1/52; for removing dissolved compounds C02F1/58
• CPC Class Codes: C02F 11/121 by mechanical de-watering
• CPC Class Codes: C02F 11/13 by heating
• CPC Class Codes: C02F 2305/06 Nutrients for stimulating the growth of microorganisms
• CPC Class Codes: C02F 3/12 Activated sludge processes
• CPC Class Codes: C02F 3/1268 Membrane bioreactor systems
• CPC Class Codes: C02F 3/32 characterised by the animals or plants used, e.g. algae
• CPC Class Codes: C02F 3/327 characterised by animals and plants
• CPC Class Codes: C12M 21/02 Photobioreactors culturing algae A01G33/00, A01H4/001, C12N1/12
• CPC Class Codes: C12M 33/00 Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus chemical or physical laboratory apparatus in general B01L, devices for taking cell samples A61B10/0045, withdrawing or distributing predetermined quantities of fluid B01L99/00
• CPC Class Codes: C12M 47/02 Separating microorganisms from the culture medium; Concentration of biomass separating microorganisms from their culture media C12N1/02
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel
• CPC Class Codes: Y02W 10/10 Biological treatment of water, waste water, or sewage
• CPC Class Codes: Y02W 10/37 using solar energy

Abstract

A wastewater treatment method includes the steps of supplying a wastewater influent having nutrients for a population of phototactic heterotrophic microorganisms to an aerated bioreactor. At least a portion of the nutrients is converted into the phototactic heterotrophic microorganism population. The wastewater influent and the phototactic heterotrophic microorganism population together form a bioreactor effluent that is transferred to a light clarifier. The bioreactor effluent is concentrated in the light clarifier to form a low-solids effluent and a high-solids effluent. The concentration is performed by inducing the population of phototactic heterotrophic microorganisms to phototactically self concentrate through exposure to a light having at least one of an intensity and a wavelength sufficient to cause the phototactic heterotrophic microorganism population to migrate away from a source of the light. The high-solids effluent is then dewatered to form a concentrated biomass paste. The low-solids effluent is further processed to form a treated water stream.

Claims

1. A wastewater treatment and biomass cultivation method, the method comprising the steps of:

providing an aerated bioreactor supporting a population of a phototactic heterotrophic microorganism;

supplying to the aerated bioreactor a wastewater influent having nutrients and an organic carbon substrate for growth of the phototactic heterotrophic microorganism population;

cultivating the phototactic heterotrophic microorganism population to convert at least a portion of the nutrients and the organic carbon substrate into the phototactic heterotrophic microorganism population, the wastewater influent and the phototactic heterotrophic microorganism population together forming a bioreactor effluent;

transferring the bioreactor effluent from the bioreactor to a light clarifier;

concentrating the bioreactor effluent in the light clarifier to form a low-solids effluent and a high-solids effluent, the concentration performed by inducing the phototactic heterotrophic microorganism population to phototactically self concentrate by exposing the phototactic heterotrophic microorganism population to a source of light having at least one of an intensity and a wavelength sufficient to cause the phototactic heterotrophic microorganism population to migrate away from the source of light, wherein the phototactic heterotrophic microorganism population is concentrated to form the high-solids effluent;

dewatering the high-solids effluent to form a concentrated biomass paste; and

processing the low-solids effluent to form a treated water stream.

2. The wastewater treatment method of claim 1, further comprising the steps of diverting a first portion of the high-solids effluent to the bioreactor and at least one of dewatering and drying a second portion of the high-solids effluent to provide a concentrated biomass paste.

3. The wastewater treatment method of claim 1, wherein the step of dewatering the high-solids effluent includes one of centrifuging, belt pressing, and heated screw pressing the high-solids effluent.

4. The wastewater treatment method of claim 1, further comprising the steps of hydrolyzing the biomass paste and converting the hydrolyzed paste into ethanol.

5. The wastewater treatment method of claim 1, wherein the phototactic heterotrophic microorganism is one of a phototactic protist and a phototactic alga exhibiting negative phototaxis.

6. The wastewater treatment method of claim 5, wherein the phototactic heterotrophic microorganism is a Euglenoid.

7. The wastewater treatment method of claim 1, wherein the wastewater influent includes a biological oxygen demand (BOD), a concentration of nitrogen, and a concentration of phosphorus sufficient to grow the phototactic heterotrophic microorganism population.

8. The wastewater treatment method of claim 1, wherein the wastewater influent is supplemented with a secondary carbon source including at least one of glycerin and a biodiesel by-product.

9. The wastewater treatment method of claim 1, wherein the biomass paste is fractionated by at least one of acid fractionation, base fractionation, high pressure fractionation, and mechanical fractionation to form a paramylon fraction and a non-paramylon fraction.

10. The wastewater treatment method of claim 9, wherein the non-paramylon fraction includes a wax-ester fraction.

11. The wastewater treatment method of claim 1, furthering including a step of adjusting a pH of the bioreactor effluent to favor growth of the phototactic heterotrophic microorganism population over growth of competitor microorganisms.

12. The wastewater treatment method of claim 1, wherein the source of light to which the phototactic heterotrophic microorganism population is exposed to cause the self concentration has a wavelength from about 400 nm to about 550 nm.

13. The wastewater treatment method of claim 1, wherein the phototactic heterotrophic microorganism is exposed to another source of light while in the aerated bioreactor, the another source of light having at least one of an intensity and a wavelength sufficient to promote a growth of the phototactic heterotrophic microorganism population.

14. A system for cultivating biomass, comprising:

an aerated bioreactor supporting a population of a phototactic heterotrophic microorganism and configured to receive an influent stream having nutrients and an organic carbon substrate for growth of the phototactic heterotrophic microorganism population, the influent stream and the phototactic heterotrophic microorganism population together forming a bioreactor effluent.

15. The system of claim 14, further comprising a light clarifier in fluid communication with the aerated bioreactor, the light clarifier configured to receive the bioreactor effluent and expose the phototactic heterotrophic microorganism population to a source of light having at least one of an intensity and a wavelength sufficient to cause the phototactic heterotrophic microorganism population to migrate away from the source of light, wherein the phototactic heterotrophic microorganism population is concentrated to form a high-solids effluent and leave a low-solids effluent.

16. The system of claim 15, wherein the source of light includes an artificial lighting system disposed adjacent the light clarifier and providing a light having at least one of an intensity and a wavelength sufficient to cause the phototactic heterotrophic microorganism population to self concentrate, and wherein the system further includes another artificial light system disposed adjacent the aerated bioreactor and providing another light having at least one of an intensity and a wavelength sufficient to promote a growth of the phototactic heterotrophic microorganism population and not cause the phototactic heterotrophic microorganism population to self concentrate in the aerated bioreactor.

17. The system of claim 14, further comprising a pH controller in fluid communication with the aerated bioreactor, the pH controller configured to modulate a pH of the bioreactor effluent, the modulated pH being conducive to growth of the population of the phototactic heterotrophic microorganism population and militating against growth of competitor microorganisms.

18. The system of claim 14, further comprising a biomass concentrating subsystem, the biomass concentrating subsystem including at least one of a lysing subsystem, a flocculation subsystem, a membrane filtration subsystem, and a dissolved air flotation subsystem to separate solids including the heterotrophic microorganism population and liquid from the bioreactor effluent.

19. A biomass cultivation method, the method comprising the steps of:

providing an aerated bioreactor supporting a population of a phototactic heterotrophic microorganism;

supplying to the aerated bioreactor an influent stream having nutrients and an organic carbon substrate for the phototactic heterotrophic microorganism population;

cultivating the phototactic heterotrophic microorganism population to convert at least a portion of the nutrients and organic carbon substrate into the phototactic heterotrophic microorganism population, the influent stream and the phototactic heterotrophic microorganism population together forming a bioreactor effluent; and

at least one of

concentrating the bioreactor effluent in the light clarifier to form a low-solids effluent and a high-solids effluent, the concentration performed by inducing the phototactic heterotrophic microorganism population to phototactically self concentrate by exposing the phototactic heterotrophic microorganism population to a source of light having at least one of an intensity and a wavelength sufficient to cause the phototactic heterotrophic microorganism population to migrate away from the source of light, wherein the phototactic heterotrophic microorganism population is concentrated to form the high-solids effluent,

treating the bioreactor effluent with a base to form the low-solids effluent and the high-solids effluent, wherein the base lyses at least a portion of the heterotrophic microorganism population in the bioreactor effluent, and wherein the lysed heterotrophic microorganism population precipitates to form the high-solids effluent,

introducing a flocculent to the bioreactor effluent to induce a rapid settling of at least a portion of the heterotrophic microorganism population to form the low-solids effluent and the high-solids effluent,

filtering the bioreactor effluent through a membrane filter to remove at least a portion of the phototactic heterotrophic microorganism population to form the low-solids effluent and the high-solids effluent, and

separating the bioreactor effluent into the low-solids effluent and the high-solids effluent by dissolved air flotation.

20. The biomass cultivation method of claim 19, further comprising the step of at least one of dewatering and drying the high-solids effluent to form a concentrated biomass paste.