Methods of selective extraction and fractionation of algal products
First Claim
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1. A method of selectively separating products from a wet algal biomass comprised of intact algal cells, the method comprising:
- a. providing a wet algal biomass;
b. adding a first solvent set, to generate a first extraction mixture comprising a first liquid phase and a first solid phase, wherein the first extraction mixture comprises intact algal cells and is made up of about 50-65% of the first solvent set;
c. separating at least a portion of the first liquid phase from the first solid phase;
d. combining the first solid phase with a second solvent set, to generate a second extraction mixture comprising a second liquid phase and a second solid phase, wherein the second extraction mixture comprises intact algal cells and is made up of about 75-85% of the second solvent set;
e. separating at least a portion of the second liquid phase from the second solid phase;
f. combining the second solid phase with a third solvent set, to generate a third extraction mixture comprising a third liquid phase and a third solid phase, wherein the third extraction mixture comprises intact algal cells and is made up of about 85-95% of the third solvent set; and
g. separating at least a portion of the third liquid phase from the third solid phase, wherein the first liquid phase has a higher protein concentration than the second and third liquid phases, the second liquid phase has a higher polar lipids concentration than the first and third liquid phases, and the third liquid phase has a higher neutral lipids concentration than the first and second liquid phases.
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Abstract
Methods for selective extraction and fractionation of algal lipids and algal products are disclosed. A method of selective removal of products from an algal biomass provides for single and multistep extraction processes which enable efficient separation of algal components. Among these components are neutral lipids synthesized by algae, which are extracted by the methods disclosed herein for the production of renewable fuels.
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Citations
22 Claims
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1. A method of selectively separating products from a wet algal biomass comprised of intact algal cells, the method comprising:
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a. providing a wet algal biomass; b. adding a first solvent set, to generate a first extraction mixture comprising a first liquid phase and a first solid phase, wherein the first extraction mixture comprises intact algal cells and is made up of about 50-65% of the first solvent set; c. separating at least a portion of the first liquid phase from the first solid phase; d. combining the first solid phase with a second solvent set, to generate a second extraction mixture comprising a second liquid phase and a second solid phase, wherein the second extraction mixture comprises intact algal cells and is made up of about 75-85% of the second solvent set; e. separating at least a portion of the second liquid phase from the second solid phase; f. combining the second solid phase with a third solvent set, to generate a third extraction mixture comprising a third liquid phase and a third solid phase, wherein the third extraction mixture comprises intact algal cells and is made up of about 85-95% of the third solvent set; and g. separating at least a portion of the third liquid phase from the third solid phase, wherein the first liquid phase has a higher protein concentration than the second and third liquid phases, the second liquid phase has a higher polar lipids concentration than the first and third liquid phases, and the third liquid phase has a higher neutral lipids concentration than the first and second liquid phases. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A method of selectively separating products from a wet algal biomass comprised of intact algal cells, the method comprising:
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a. providing a wet algal biomass; b. adding a first solvent set, to generate a first extraction mixture comprising a first liquid phase and a first solid phase, wherein the first extraction mixture comprises intact algal cells and is made up of at least about 50% of the first solvent set; c. separating at least a portion of the first liquid phase from the first solid phase; d. combining the first solid phase with a second solvent set, to generate a second extraction mixture comprising a second liquid phase and a second solid phase, wherein the second extraction mixture comprises intact algal cells and is made up of at least about 75% of the second solvent set; e. separating at least a portion of the second liquid phase from the second solid phase; f. combining the second solid phase with a third solvent set, to generate a third extraction mixture comprising a third liquid phase and a third solid phase, wherein the third extraction mixture comprises intact algal cells and is made up of at least about 85% of the third solvent set; and g. separating at least a portion of the third liquid phase from the third solid phase, wherein the first liquid phase has a higher protein concentration than the second and third liquid phases, the second liquid phase has a higher polar lipids concentration than the first and third liquid phases, and the third liquid phase has a higher neutral lipids concentration than the first and second liquid phases. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22)
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Specification