Multi-well system
First Claim
1. A multi-well system for characterizing cells, comprising:
- a tray comprising a plurality of wells, each of the wells comprising a side wall, a bottom and an open top;
a first tray top comprising a plurality of areas, each of the areas corresponding uniquely with each well of the tray;
a protein binding agent bound to each of the plurality of areas of the first tray top; and
a second tray top comprising a plurality of areas, each of the areas corresponding uniquely with each well of the tray, wherein there are oligonucleotide tags bound to the plurality of areas of the second tray top, and wherein the oligonucleotide tags are specific tags that correlate nucleic acids obtained from cells in the wells with a physical location of the wells, are specific to the well of interest and thereby allow pooling of the nucleic acids obtained from cells in the wells for subsequent analysis by ultra-high throughput sequencing.
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Accused Products
Abstract
A multi-well system is described which is comprised of a tray comprising a plurality of wells which may include thousands, tens of thousands or even hundreds of thousands of wells or more. Each of the wells has a unique address. The system includes a tray top comprised of a plurality of areas wherein each of the areas corresponds uniquely to each of the wells and includes a unique address. The system may include a second tray top also comprised of a plurality of areas with unique addresses which uniquely correspond to each of the wells. The areas on the tops have compounds bound to those areas which compounds bind to components present in the wells and are used to obtain specific material in each well for analysis.
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Citations
14 Claims
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1. A multi-well system for characterizing cells, comprising:
- a tray comprising a plurality of wells, each of the wells comprising a side wall, a bottom and an open top;
a first tray top comprising a plurality of areas, each of the areas corresponding uniquely with each well of the tray;
a protein binding agent bound to each of the plurality of areas of the first tray top; and
a second tray top comprising a plurality of areas, each of the areas corresponding uniquely with each well of the tray, wherein there are oligonucleotide tags bound to the plurality of areas of the second tray top, and wherein the oligonucleotide tags are specific tags that correlate nucleic acids obtained from cells in the wells with a physical location of the wells, are specific to the well of interest and thereby allow pooling of the nucleic acids obtained from cells in the wells for subsequent analysis by ultra-high throughput sequencing. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
- a tray comprising a plurality of wells, each of the wells comprising a side wall, a bottom and an open top;
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11. A multi-well system, comprising:
- a tray comprised of a frame and a plurality of wells wherein a plurality of the plurality of the wells has bound to its surface an antibody-producing cell;
a first tray top comprising a plurality of areas, each of the areas corresponding uniquely with each of the wells of the tray;
an antibody binding agent bound to each of the plurality of areas of the first tray top;
a second tray top comprising a plurality of areas, each of the areas corresponding uniquely with each well of the tray; and
oligonucleotide tags bound to each of the plurality of areas of the second tray top, wherein the oligonucleotide tags are specific tags that correlate nucleic acids obtained from cells in the wells to a physical location of the wells, are specific to the well of interest and thereby allow pooling of the nucleic acids obtained from the wells for subsequent analysis by ultra-high throughput sequencing. - View Dependent Claims (12, 13)
- a tray comprised of a frame and a plurality of wells wherein a plurality of the plurality of the wells has bound to its surface an antibody-producing cell;
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14. A device to allow optical inspection of cells in a multi-well system, where the multi-well system comprises:
- a tray comprising a plurality of wells, each of the wells comprising a side wall, a bottom and an open top;
a first tray top comprising a plurality of areas, each of the areas corresponding uniquely with each well of the tray;
an antibody binding agent bound to each of the plurality of areas of the first tray top;
a second tray top comprising a plurality of areas, each of the areas corresponding uniquely with each well of the tray; and
an oligonucleotide tag bound to each of the plurality of areas of the second tray top, wherein the oligonucleotide tags are specific tags that correlate the nucleic acids obtained from cells in the wells with a physical location of the wells, are specific to the well of interest and thereby allow pooling of nucleic acids obtained from the wells for subsequent analysis by ultra-high throughput sequencing, wherein the device is capable of aligning the tray and the first tray top and the tray and the second tray top.
- a tray comprising a plurality of wells, each of the wells comprising a side wall, a bottom and an open top;
Specification