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Method of performing an immunoassay in blood

  • US 8,309,364 B2
  • Filed: 02/18/2010
  • Issued: 11/13/2012
  • Est. Priority Date: 09/10/2003
  • Status: Expired due to Term
First Claim
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1. A method of performing an immunoassay for an analyte in blood and correcting for the hematocrit of the sample, comprising:

  • (a) contacting an immunosensor with a blood sample,(b) contacting said blood sample with a bulk conductivity sensor and measuring its resistance,(c) contacting the immunosensor and conductivity sensor with an aqueous solution having known conductivity and containing reagents sufficient to generate a detectable product related to the amount of analyte bound to the immunosensor by antibody-antigen binding, wherein the reagents comprise a label that reacts with a substrate to generate the detectable product,(d) measuring the signal at the immunosensor generated by said product,(e) converting by means of an algorithm the signal from the immunosensor to an analyte concentration,(f) calculating the hematocrit value of the blood sample from the measured resistance of the blood sample and the known conductivity of the aqueous solution, and(g) correcting the calculated analyte concentration for the hematocrit of the sample.

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