Antisense antibacterial method and compound
First Claim
1. A method of inhibiting the growth of pathogenic bacterial cells, comprising exposing the bacterial cells to a growth-inhibiting amount of a substantially uncharged antisense morpholino oligomer having a sequence consisting of SEQ ID NO:
- 109,wherein said morpholino oligomer is composed of morpholino subunits and phosphorodiamidate intersubunit linkages joining a morpholino nitrogen of one subunit to a 5′
exocyclic carbon of an adjacent subunit.
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Abstract
A method and antisense compound for inhibiting the growth of pathogenic bacterial cells are disclosed. The compound contains no more than 12 nucleotide bases and has a targeting nucleic acid sequence of no fewer than 10 bases in length that is complementary to a target sequence containing or within 10 bases, in a downstream direction, of the translational start codon of a bacterial mRNA that encodes a bacterial protein essential for bacterial replication. The compound binds to a target mRNA with a Tm of between 50° to 60° C. The relatively short antisense compounds are substantially more active than conventional antisense compounds having a targeting base sequence of 15 or more bases.
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Citations
5 Claims
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1. A method of inhibiting the growth of pathogenic bacterial cells, comprising exposing the bacterial cells to a growth-inhibiting amount of a substantially uncharged antisense morpholino oligomer having a sequence consisting of SEQ ID NO:
- 109,
wherein said morpholino oligomer is composed of morpholino subunits and phosphorodiamidate intersubunit linkages joining a morpholino nitrogen of one subunit to a 5′
exocyclic carbon of an adjacent subunit. - View Dependent Claims (2, 3, 4, 5)
- 109,
Specification