Optically-detectable enzyme substrates and their method of use
First Claim
1. A method for determining the presence or absence of β
- -lactamase enzyme in a sample, the method comprising;
a) contacting the sample with a β
-lactamase substrate to form a contacted sample;
b) incubating the contacted sample for a sufficient amount of time for a β
-lactamase enzyme in the contacted sample to cleave the β
-lactamase substrate to form an incubated sample;
c) illuminating the incubated sample with an appropriate wavelength to obtain an illuminated sample ; and
d) observing the illuminated sample whereby the presence or absence of β
-lactamase enzyme in the illuminated sample is determinedwherein the β
-lactamase substrate has a structure of one of the following formulae;
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Accused Products
Abstract
The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a β-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a β-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a β-lactmase or instructions for using the components in an assay.
107 Citations
4 Claims
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1. A method for determining the presence or absence of β
- -lactamase enzyme in a sample, the method comprising;
a) contacting the sample with a β
-lactamase substrate to form a contacted sample;b) incubating the contacted sample for a sufficient amount of time for a β
-lactamase enzyme in the contacted sample to cleave the β
-lactamase substrate to form an incubated sample;c) illuminating the incubated sample with an appropriate wavelength to obtain an illuminated sample ; and d) observing the illuminated sample whereby the presence or absence of β
-lactamase enzyme in the illuminated sample is determinedwherein the β
-lactamase substrate has a structure of one of the following formulae;
- -lactamase enzyme in a sample, the method comprising;
-
2. A method for determining the presence or absence of β
- -lactamase enzyme in a sample, the method comprising;
a) contacting the sample with a β
-lactamase substrate to form a contacted sample;b) incubating the contacted sample for a sufficient amount of time for a β
-lactamase enzyme in the contacted sample to cleave the β
-lactamase substrate to form an incubated sample;c) illuminating the incubated sample with an appropriate wavelength to obtain an illuminated sample; and d) observing the illuminated sample whereby the presence or absence of β
-lactamase enzyme in the illuminated sample is determined, wherein the β
-lactamase substrate has a structure of the formula;
- -lactamase enzyme in a sample, the method comprising;
-
3. A method for determining the presence or absence of β
- -lactamase enzyme in a sample, the method comprising;
a) contacting the sample with a β
-lactamase substrate to form a contacted sample;b) incubating the contacted sample for a sufficient amount of time for a β
-lactamase enzyme in the contacted sample to cleave the β
-lactamase substrate to form an incubated sample;c) illuminating the incubated sample with an appropriate wavelength to obtain an illuminated sample; and d) observing the illuminated sample whereby the presence or absence of β
-lactamase enzyme in the illuminated sample is determined, wherein the β
-lactamase substrate has a structure of the formula;
- -lactamase enzyme in a sample, the method comprising;
-
4. A method for determining the presence or absence of β
- -lactamase enzyme in a sample, the method comprising;
a) contacting the sample with a β
-lactamase substrate to form a contacted sample;b) incubating the contacted sample for a sufficient amount of time for a β
-lactamase enzyme in the contacted sample to cleave the β
-lactamase substrate to form an incubated sample;c) illuminating the incubated sample with an appropriate wavelength to obtain an illuminated sample; and d) observing the illuminated sample whereby the presence or absence of β
-lactamase enzyme in the illuminated sample is determined, wherein the β
-lactamase substrate has a structure of the formula;
- -lactamase enzyme in a sample, the method comprising;
Specification