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Optically-detectable enzyme substrates and their method of use

  • US 8,318,450 B2
  • Filed: 06/22/2011
  • Issued: 11/27/2012
  • Est. Priority Date: 01/21/2004
  • Status: Expired due to Fees
First Claim
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1. A method for determining the presence or absence of β

  • -lactamase enzyme in a sample, the method comprising;

    a) contacting the sample with a β

    -lactamase substrate to form a contacted sample;

    b) incubating the contacted sample for a sufficient amount of time for a β

    -lactamase enzyme in the contacted sample to cleave the β

    -lactamase substrate to form an incubated sample;

    c) illuminating the incubated sample with an appropriate wavelength to obtain an illuminated sample ; and

    d) observing the illuminated sample whereby the presence or absence of β

    -lactamase enzyme in the illuminated sample is determinedwherein the β

    -lactamase substrate has a structure of one of the following formulae;

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