Process for immobilization of protein catalysts, product, and use

• US 8,318,466 B2
• Filed: 11/25/2009
• Issued: 11/27/2012
• Est. Priority Date: 05/07/2004
• Status: Expired due to Fees

• Current Assignee: GM Global Technology Operations LLC (General Motors Company)
• Original Assignee: GM Global Technology Operations LLC (General Motors Company)
• Inventors: Dziedzic, Daniel, Johnson, John T, Gross, Kenneth B
• Primary Examiner(s): Ha, Julie
• Application Number:
  US12/626,159
• Publication Number:
  US 20100068784A1
• Time in Patent Office: 1,098 Days
• Field of Search: None
• US Class Current: 435/176
• CPC Class Codes: C12N 11/06 attached to the carrier via a bridging agent
• CPC Class Codes: C12N 9/88 Lyases (4.)
• CPC Class Codes: Y10T 428/31507 Of polycarbonate
• CPC Class Codes: Y10T 428/31511 Of epoxy ether
• CPC Class Codes: Y10T 428/31551 Of polyamidoester [polyurethane, polyisocyanate, polycarbamate, etc.]
• CPC Class Codes: Y10T 428/31725 Of polyamide
• CPC Class Codes: Y10T 428/31786 Of polyester [e.g., alkyd, etc.]
• CPC Class Codes: Y10T 428/31931 Polyene monomer-containing
• CPC Class Codes: Y10T 428/31938 Polymer of monoethylenically unsaturated hydrocarbon

Abstract

An immobilized protein catalyst is prepared by applying an adhesive to a polymeric support, applying a layer of a globular protein over the layer of adhesive, binding a crosslinking agent to the protein layer, and binding the protein catalyst by reaction with the crosslinking agent.

Claims

1. An immobilized protein catalyst, comprising

a polymeric support,

a polymeric adhesive layer over the polymeric support,

a layer of a globular protein over the polymeric adhesive layer,

a crosslinking agent attached to the globular protein layer, and

a protein catalyst reacted onto the layer of globular protein by the crosslinking agent.

2. An immobilized protein catalyst according to claim 1, wherein the polymeric support comprises a member selected from the group consisting of polyethylene, polypropylene, polybutadiene, polyamide, polycarbonate, vinyl polymers, polypropylene glycol, polyesters, phenolic polymers, epoxy polymers, polyurethanes, acrylic polymers, styrene polymers and copolymers, and combinations thereof.

3. An immobilized protein catalyst according to claim 1, wherein the globular protein comprises an albumin.

4. An immobilized protein catalyst according to claim 1, wherein the globular protein comprises ovalbumin.

5. An immobilized protein catalyst according to claim 1, wherein the protein catalyst is reacted onto the layer of globular protein by glutaraldehyde.

6. An immobilized protein catalyst according to claim 1, wherein the protein catalyst comprises carbonic anhydrase.

7. A method of hydrating carbon dioxide, comprising contacting carbon dioxide with water in the presence of the immobilized carbonic anhydrase according to claim 6.

8. An immobilized protein catalyst according to claim 1, wherein the polymeric adhesive layer comprises a one-component solvent based adhesive.