Co-culturing mammalian embryonic stem cells with human foreskin fibroblasts
First Claim
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1. A method of maintaining mammalian pluripotent stem cells in an undifferentiated state comprising co-culturing the mammalian pluripotent stem cells with a human foreskin fibroblast feeder cell line, wherein said mammalian pluripotent stem cells are capable of forming teratomas containing cells of the endoderm, mesoderm and ectoderm germ layers.
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Abstract
A cell culture comprising human foreskin cells, the human foreskin cells being capable of maintaining stem cells in an undifferentiated state when co-cultured therewith.
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18 Claims
- 1. A method of maintaining mammalian pluripotent stem cells in an undifferentiated state comprising co-culturing the mammalian pluripotent stem cells with a human foreskin fibroblast feeder cell line, wherein said mammalian pluripotent stem cells are capable of forming teratomas containing cells of the endoderm, mesoderm and ectoderm germ layers.
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11. A cell culture comprising:
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(i) mammalian pluripotent stem cells, wherein said mammalian pluripotent stem cells are capable of forming teratomas containing cells of the endoderm, mesoderm and ectoderm germ layers; and (ii) human foreskin fibroblast cells capable of maintaining said mammalian pluripotent stem cells in an undifferentiated state. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18)
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Specification