Miniaturized fluid delivery and analysis system

• US 8,323,887 B2
• Filed: 08/16/2006
• Issued: 12/04/2012
• Est. Priority Date: 09/27/2002
• Status: Active Grant

• Current Assignee: Chi-Chen Chen, James Russell Webster, Ping Chang, Rong-I Hong, Shaw-Tzuv Wang
• Original Assignee: Chi-Chen Chen, James Russell Webster, Ping Chang, Rong-I Hong, Shaw-Tzuv Wang
• Inventors: Webster, James Russell, Chang, Ping, Wang, Shaw-Tzuv, Chen, Chi-Chen, Hong, Rong-I
• Primary Examiner(s): Kapushoc, Stephen
• Assistant Examiner(s): BHAT, NARAYAN KAMESHWAR
• Application Number:
  US11/505,793
• Publication Number:
  US 20070020148A1
• Time in Patent Office: 2,302 Days
• Field of Search: 435/6.1, 435/7.9, 435/91.2, 435/287.2, 422/181, 436/180, 536/24.3
• US Class Current: 435/6.1
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/0883 Serpentine channels
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0605 check valves
• CPC Class Codes: B01L 2400/0638 membrane valves, flap valves
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: F04B 43/043 Micropumps
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

The present invention provides a method for combining a fluid delivery system with an analysis system for performing immunological or other chemical of biological assays. The method comprises a miniature plastic fluidic cartridge containing a reaction chamber with a plurality of immobilized species, a capillary channel, and a pump structure along with an external linear actuator corresponding to the pump structure to provide force for the fluid delivery. The plastic fluidic cartridge can be configured in a variety of ways to affect the performance and complexity of the assay performed.

Claims

1. A method of performing DNA hybridization analysis, comprising the steps of:

(a) immobilizing a plurality of DNA probes in a reaction chamber defined in a fluidic cartridge, wherein the fluid cartridge comprises a first substrate, a second substrate and a flexible intermediate interlayer sealedly interfaced between said first substrate and said second substrate to form therein one or more channels of capillary dimensions within the first substrate and the second substrate on both sides of flexible intermediate interlayer;

a plurality of fluid reservoirs, a pump chamber, a reaction chamber, and a port formed at least partially in said first substrate or said second substrate of said fluidic cartridge, and wherein the one or more channels connect the fluid reservoir to the pump chamber, the pump chamber to the reaction chamber, and the reaction chamber to the port;

a fluid flow controlling structure, formed in said fluidic cartridge, restricting a flow of a fluid in one direction only from said fluid reservoir to said reaction chamber via said one or more channels and said pump chamber; and

a linear actuator providing a pumping action in said pump chamber to push said fluid to flow from said fluid reservoir to said reaction chamber via said pump chamber and said one or more channels, wherein the said fluid flow controlling structure comprises a first passive check valve and a second passive check valve in said fluidic cartridge to restrict said fluid to flow from one of said one or more channels in said second substrate to another one of said one or more channels in said first substrate by bending of said pump interlayer diaphragm so as to control said fluid flowing from said fluid reservoir to said port, (b) placing a fluid sample containing one or more populations of fluorescently tagged, amplified DNA of unknown sequence in a sample fluid reservoir in said fluidic cartridge;

(c) placing a first stringency wash buffer in a first wash buffer fluid reservoir in said fluidic cartridge;

(d) placing a second stringency wash buffer in a second wash buffer fluid reservoir in said fluidic cartridge;

(e) maintaining the reaction chamber in a constant temperature;

(f) pumping said fluid sample from said sample reservoir to a circulation fluid reservoir in said fluidic cartridge and circulating said fluid sample through said reaction chamber for a predetermined hybridization time;

(g) pumping out said fluid sample from said circulation reservoir and said reaction chamber;

(h) pumping said first stringency wash buffer from said first wash buffer reservoir to said circulation reservoir and circulating said first stringency wash buffer through said reaction chamber for a first predetermined wash time;

(i) pumping out said first stringency wash buffer from said circulation reservoir and said reaction chamber;

(j) pumping said second stringency wash buffer rom said second wash buffer reservoir to said circulation reservoir and circulating said second stringency wash buffer through said reaction chamber for a second predetermined wash time;

(k) pumping out said second stringency wash buffer from said circulation reservoir and said reaction chamber; and

(i) achieving a DNA hybridization;

wherein in said pumping steps (f) to (k), said fluid sample and said first stringency wash buffer, and second stringency wash buffer are pumped by a pumping action in at least a pump chamber defined in said fluidic cartridge wherein said pumping action is provided by a linear actuator so as to pump said fluid sample and said first stringency wash buffer, and second stringency wash buffer to flow from said sample reservoir, said first wash buffer reservoir, said second wash buffer reservoir through said circulation reservoir and said reaction chamber via said one or more channels;

wherein said pump chamber has a substrate chamber formed in said first substrate and a hole formed in said second substrate to free said interlayer to act as a pump interlayer diaphragm, wherein said linear actuator moves in said hole to bend said pump interlayer diaphragm and therefore provides a necessary force to deform said pump interlayer diaphragm to provide said pumping action in said at least a pump chamber to pump said fluid sample and said first stringency wash buffer, and second stringency wash buffer from said sample reservoir, said first wash buffer reservoir, and said second wash buffer reservoir-to flow through said circulation reservoir and said reaction chamber via said one or more channels.

2. The method, as recited in claim 1, wherein said DNA hybridization is achieved by fluorescent imaging.

3. The method, as recited in claim 1, wherein said DNA hybridization is achieved by colorimetric detection.

4. The method, as recited in claim 1, wherein said DNA hybridization is achieved by luminescence detection.

5. The method, as recited in claim 1, wherein said DNA hybridization is achieved by biotin-streptavidin-enzyme detection.