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Microfluidic system for amplifying and detecting polynucleotides in parallel

DC CAFC
  • US 8,323,900 B2
  • Filed: 02/25/2011
  • Issued: 12/04/2012
  • Est. Priority Date: 03/24/2006
  • Status: Active Grant
First Claim
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1. An apparatus, comprising:

  • a plurality of multi-lane microfluidic cartridges, each lane comprising a PCR reaction zone;

    a plurality of receiving bays, each receiving bay configured to receive one of the plurality of microfluidic cartridges;

    each PCR reaction zone comprising a separately controllable heat source thermally coupled thereto, wherein the heat source thermal cycles the PCR reaction zone to carry out PCR on a polynucleotide-containing sample in the PCR reaction zone and maintains a substantially uniform temperature throughout the PCR reaction zone during each cycle;

    a detector configured to detect the presence of an amplification product in one or more PCR reaction zones; and

    a processor coupled to the detector and the heat sources, configured to control heating of one or more PCR reaction zones by the heat sources.

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